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Updated: Sep 1, 2025

LERLIC-MS/MS for In-depth Characterization and Quantification of Glutamine and Asparagine Deamidation in Shotgun Proteomics
Published on: April 9, 2017
First Experimental Evidence for Reversibility of Ammonia Loss from Asparagine
Jijing Wang1, Sergey Rodin1,2, Amir Ata Saei1,3
1Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 65 Stockholm, Sweden.
Abstract:
Ammonia loss from L-asparaginyls is a nonenzymatic reaction spontaneously occurring in all proteins and eventually resulting in damaging isoaspartate residues that hamper protein function and induce proteinopathy related to aging. Here, we discuss theoretical considerations supporting the possibility of a full repair reaction and present the first experimental evidence of its existence. If confirmed, the true repair of L-asparaginyl deamidation could open new avenues for preventing aging and neurodegenerative diseases.
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