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Updated: Sep 1, 2025

Investigating Aortic Valve Calcification via Isolation and Culture of T Lymphocytes using Feeder Cells from Irradiated Buffy Coat
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PTP1B Inhibition Improves Mitochondrial Dynamics to Alleviate Calcific Aortic Valve Disease Via Regulating

Feng Liu1,2, Jinyong Chen1,2, Wangxing Hu1,2

  • 1Department of Cardiology of The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

JACC. Basic to Translational Science
|August 12, 2022
PubMed

Insights

Protein tyrosine phosphatase 1B (PTP1B) inhibition shows promise for treating calcific aortic valve disease (CAVD). Targeting PTP1B may prevent aortic valve calcification by preserving mitochondrial function.

Area of Science:

  • Biochemistry
  • Cardiovascular Biology
  • Pharmacology

Background:

  • Calcific aortic valve disease (CAVD) lacks effective pharmacological treatments.
  • Protein tyrosine phosphatase 1B (PTP1B) is implicated in disease pathogenesis.

Purpose of the Study:

  • To investigate the role of PTP1B in CAVD.
  • To evaluate the therapeutic potential of PTP1B inhibition in CAVD.

Main Methods:

  • Assessed PTP1B expression in human calcified aortic valves.
  • Examined the effects of PTP1B inhibition on valvular interstitial cells and LDLR-/- mice.
  • Investigated PTP1B's interaction with OPA1 in mitochondrial homeostasis and osteogenesis.

Main Results:

  • PTP1B was upregulated in calcified human aortic valves.
  • PTP1B inhibition reduced the fibrocalcific response in vitro and in vivo.
  • PTP1B regulates mitochondrial homeostasis via OPA1 isoform transition, impacting valvular interstitial cell osteogenesis.

Conclusions:

  • PTP1B plays a critical role in CAVD pathogenesis.
  • PTP1B inhibition demonstrates therapeutic potential for CAVD.
  • Targeting PTP1B may offer a novel strategy for preventing aortic valve calcification.
Keywords:
CAVD, calcific aortic valve diseaseHFD, high-fat dietL-OPA1, long form of OPA1OPA1, optic atrophy 1PTP1B, protein tyrosine phosphatase 1BS-OPA1, short form of OPA1TGFβ, transforming growth factor βVIC, valvular interstitial cellcalcific aortic valve diseasemitochondrial biogenesisoptic atrophy 1protein tyrosine phosphatase 1Bvalvular interstitial cellsβ-GA, β-glycerophosphate acid

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