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Immunogold Electron Microscopy01:20

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Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
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Protein Kinase C-delta Inhibitor Peptide Formulation using Gold Nanoparticles
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Biologically Derived Gold Nanoparticles and Their Applications.

Arpita Roy1, Chetan Pandit2, Amel Gacem3

  • 1Department of Biotechnology, School of Engineering & Technology, Sharda University, Greater Noida, India.

Bioinorganic Chemistry and Applications
|August 12, 2022
PubMed
Summary

Biological synthesis offers an eco-friendly method for producing gold nanoparticles. This review summarizes current research on green synthesis of gold nanoparticles and their diverse applications.

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Area of Science:

  • Materials Science
  • Biotechnology
  • Nanotechnology

Background:

  • Traditional nanoparticle synthesis methods face limitations like chemical contamination and high energy consumption.
  • There is a growing demand for sustainable, cost-effective, and environmentally friendly nanoparticle production techniques.
  • Biological synthesis has emerged as a promising alternative for nanoparticle fabrication.

Purpose of the Study:

  • To review contemporary research on the biosynthesis of gold nanoparticles.
  • To summarize the applications of biologically synthesized gold nanoparticles across various fields.

Main Methods:

  • Utilizing biological agents such as plants, fungi, and bacteria for nanoparticle synthesis.
  • Leveraging the metabolic activities of these agents to convert metal ions into nanometals.
  • Focusing on green chemistry principles for nanoparticle production.

Main Results:

  • Biosynthesis provides a one-step, eco-friendly process for creating gold nanoparticles.
  • Various biological entities can be effectively employed for the green synthesis of gold nanoparticles.
  • Biologically synthesized gold nanoparticles exhibit potential in diverse application domains.

Conclusions:

  • Biological synthesis is a viable and sustainable approach for gold nanoparticle production.
  • Further research into biosynthesis can lead to advancements in nanotechnology and its applications.
  • The environmental benefits and efficiency of biosynthesis highlight its importance in modern science.