Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Transcriptomics of S3 segment in mice: response to type 1 diabetes, SGLT1/2 inhibition, or GLP1 receptor agonism.

American journal of physiology. Renal physiology·2026

Same author

Measuring the quality of species list governance.

Bioscience·2026

Same author

Measuring the Quality of Species List Contents.

Bioscience·2026

Same author

Effect of Subglottic Stenosis on Expiratory Sound Using Direct Noise Calculation.

Applied sciences (Basel, Switzerland)·2026

Same author

A Systematic Review of Ecological Momentary Assessment Procedures of Self-Harm (With and Without Suicidal Intent) Studies in Adolescents and Young Adults.

International journal of environmental research and public health·2026

Same author

"It feels like the world is falling on your head": Exploring the link between financial instability, employment insecurity, and suicidality.

Death studies·2025

Search research articles

Related Experiment Video

Updated: Sep 1, 2025

Microfluidic Chip Fabrication and Method to Detect Influenza

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

Ultra-rapid real-time microfluidic RT-PCR instrument for nucleic acid analysis.

Renna L Nouwairi¹, Larissa L Cunha¹, Rachelle Turiello¹

¹University of Virginia, USA. rln3bm@virginia.edu.

|August 12, 2022

Summary

This study introduces a rapid Polymerase Chain Reaction (PCR) system for quick DNA and RNA amplification. The novel instrument achieves real-time PCR in under 10 minutes, significantly reducing assay times.

More Related Videos

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Published on: February 3, 2021

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Related Experiment Videos

Last Updated: Sep 1, 2025

Microfluidic Chip Fabrication and Method to Detect Influenza

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Published on: February 3, 2021

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Area of Science:

Biotechnology
Molecular Biology
Analytical Chemistry

Background:

Polymerase Chain Reaction (PCR) is the gold standard for nucleic acid amplification.
Existing PCR instrumentation has limitations for point-of-need testing due to assay duration.
There is a need for rapid PCR systems to enable faster diagnostics.

Purpose of the Study:

To develop and present a novel instrument for ultra-fast real-time PCR.
To demonstrate rapid DNA and RNA amplification for point-of-need applications.
To reduce the time-to-result for PCR assays.

Main Methods:

A field-forward prototype instrument with custom microfluidic chips was designed.
A novel mechatronic system utilized two thermoelectric Peltiers for rapid heating and cooling (12 and 10 °C/s).
Synchronized thermal cycling and fluorescence monitoring were implemented.

Main Results:

The instrument completed 40 cycles of real-time PCR in under 10 minutes.
This represents an 84% reduction in time compared to standard 50-minute assays.
Robust amplification and detection of DNA and RNA targets were achieved.

Conclusions:

The developed rapid PCR system significantly accelerates nucleic acid amplification.
This technology enables faster, actionable information for diverse laboratory applications.
The system addresses the need for rapid, point-of-need testing solutions.