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Fixation and Sectioning01:03

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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  • 1Dr. T.N. Helm is from the Department of Dermatology, Buffalo Medical Group, Williamsville, New York. Drs. M.F. Helm and K.F. Helm are from the Department of Dermatology, Penn State Health Milton S. Hershey Medical Center, Hershey, Pennsylvania. Dr. K.F. Helm also is from the Department of Pathology.

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