Related Experiment Video
Updated: Sep 1, 2025
12:33
Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
Published on: July 28, 2017
13.0K
How do emerging long-read sequencing technologies function in transforming the plant pathology research landscape?
Islam Hamim1,2,3, Ken-Taro Sekine4, Ken Komatsu5
1Laboratory of Plant Pathology, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Japan.
Plant Molecular Biology
|August 13, 2022
Summary
Long-read sequencing (LRS) advances plant and pathogen genomics, enabling comprehensive analysis of genomes and transcriptomes. This technology revolutionizes disease management and the development of new plant varieties.
Area of Science:
- Genomics and Transcriptomics
- Plant Pathology
- Agricultural Science
Background:
- Plant and pathogen genomes/transcriptomes are complex, impacting growth, development, and disease responses.
- Second-generation sequencing has limitations in analyzing repetitive genomic regions and full-length transcripts.
- Long-read sequencing (LRS) overcomes these limitations by generating significantly longer reads.
Purpose of the Study:
- To review how LRS technologies are transforming the analysis of plant and pathogen genomes and transcriptomes.
- To highlight the benefits of LRS for studying plant-pathogen interactions and developing new cultivars.
- To explore emerging areas of interest driven by LRS capabilities.
Main Methods:
- Utilizes long-read sequencing (LRS) technologies for DNA and RNA analysis.
- Employs advanced bioinformatics tools and methods developed for long-read data.
- Focuses on applications in plant and pathogen genomics and transcriptomics.
Main Results:
- LRS enables more accurate genome assembly, especially in repetitive regions.
- Facilitates the assembly of full-length transcripts and detection of DNA/RNA alterations.
- Improves identification of transcriptome diversity, structural variations, and epigenetic marks.
- Efficiently identifies and characterizes plant effectors and pathogens.
Conclusions:
- LRS is revolutionizing plant and pathogen genomics and transcriptomics with increased accuracy and efficiency.
- It offers significant advantages for studying plant-pathogen interactions, disease control, and crop improvement.
- LRS opens new avenues for research in agriculture and plant pathology.
Related Concept Videos
03:21RNA-seq
10.3K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.3K
03:00Next-generation Sequencing
92.4K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
92.4K
01:29Modern Molecular Taxonomy
114
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
114