Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Extrinsic and Intrinsic Pathways of Hemostasis01:20

Extrinsic and Intrinsic Pathways of Hemostasis

9.0K
Blood clotting or coagulation involves extrinsic and intrinsic pathways, which ultimately merge into the common pathway, forming a fibrin clot.
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which...
9.0K
Introduction to Hemostasis01:05

Introduction to Hemostasis

8.8K
Hemostasis is a complex physiological process that prevents excessive bleeding when a blood vessel is injured. It's crucial for maintaining the integrity of the circulatory system, as it ensures that our blood remains fluid while still within the vascular network and yet clots to prevent blood loss upon vessel injury.
The three phases of hemostasis involve many clotting factors present in plasma and several substances released by platelets and injured tissue cells. It is a fast, localized,...
8.8K
Disorders of Hemostasis01:24

Disorders of Hemostasis

1.2K
Hemostasis, the process that stops bleeding after a blood vessel injury, is crucial for maintaining the integrity of the circulatory system. However, disorders of hemostasis can disrupt this delicate balance, leading to either excessive clotting or bleeding. These disorders can be broadly classified into thromboembolic disorders and bleeding disorders.
Thromboembolic Disorders
Two factors primarily cause thromboembolic conditions.
1.2K
Vaccinations01:51

Vaccinations

45.2K
Overview
45.2K
Anticoagulant Drugs: Low-Molecular-Weight Heparins01:30

Anticoagulant Drugs: Low-Molecular-Weight Heparins

867
Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
867

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interpreting protein dose trials in critical illness: a guide for the bedside clinician.

Critical care (London, England)·2026
Same author

A Household Cluster of Tick-Borne Encephalitis in Belgium in 2025: Is the Epidemiology Evolving?

Viruses·2026
Same author

Adaption of the plasmin generation assay to enhance sensitivity to plasminogen activator inhibitor-1 and establishment of sex-specific reference values in human plasma.

Research and practice in thrombosis and haemostasis·2026
Same author

Optimal delivery of enteral protein in the critically ill: A systematic review and meta-analysis of randomised controlled trials.

Clinical nutrition (Edinburgh, Scotland)·2026
Same author

Single-domain antibodies to reverse the effects of factor Xa inhibitors on coagulation tests.

Journal of thrombosis and haemostasis : JTH·2026
Same author

Pharmacotherapeutic interventions to improve postoperative sleep quality in older adult patients: a systematic review and meta-analysis.

British journal of anaesthesia·2026

Related Experiment Video

Updated: Sep 1, 2025

Author Spotlight: Advancing Immune Monitoring in Critical Care Patients Using Whole Blood Assays
06:03

Author Spotlight: Advancing Immune Monitoring in Critical Care Patients Using Whole Blood Assays

Published on: September 20, 2024

1.4K

Population-wide persistent hemostatic changes after vaccination with ChAdOx1-S.

Bas de Laat1,2, Hendrik Stragier3,4, Romy de Laat-Kremers2

  • 1Department of Functional Coagulation, Synapse Research Institute, Maastricht, Netherlands.

Frontiers in Cardiovascular Medicine
|August 15, 2022
PubMed
Summary

ChAdOx1-S vaccination was linked to altered hemostasis in health care workers. The vaccine may increase thrombosis risk through endothelial activation and a procoagulant profile.

Keywords:
COVID-19ChAdOx1-Shemostasisthrombin generationvaccination

More Related Videos

Tail Vein Transection Bleeding Model in Fully Anesthetized Hemophilia A Mice
08:13

Tail Vein Transection Bleeding Model in Fully Anesthetized Hemophilia A Mice

Published on: September 30, 2021

6.5K
Microfluidics in Assessing Platelet Function
06:47

Microfluidics in Assessing Platelet Function

Published on: November 8, 2024

1.0K

Related Experiment Videos

Last Updated: Sep 1, 2025

Author Spotlight: Advancing Immune Monitoring in Critical Care Patients Using Whole Blood Assays
06:03

Author Spotlight: Advancing Immune Monitoring in Critical Care Patients Using Whole Blood Assays

Published on: September 20, 2024

1.4K
Tail Vein Transection Bleeding Model in Fully Anesthetized Hemophilia A Mice
08:13

Tail Vein Transection Bleeding Model in Fully Anesthetized Hemophilia A Mice

Published on: September 30, 2021

6.5K
Microfluidics in Assessing Platelet Function
06:47

Microfluidics in Assessing Platelet Function

Published on: November 8, 2024

1.0K

Area of Science:

  • Hematology
  • Immunology
  • Vaccinology

Background:

  • Reports suggest a potential link between anti-SARS-CoV-2 vaccines, including ChAdOx1-S, and an increased risk of thrombosis.
  • Understanding the hemostatic and immune changes post-vaccination is crucial for assessing vaccine safety.

Purpose of the Study:

  • To investigate the hemostatic and immune system changes following ChAdOx1-S vaccination.
  • To evaluate potential mechanisms linking ChAdOx1-S vaccination to thrombotic events.

Main Methods:

  • Blood samples from 631 health care workers were analyzed post-second ChAdOx1-S vaccination.
  • Assessed hemostatic markers (D-dimer, fibrinogen, thrombin generation), endothelial function (VWF, active VWF), and immune markers (IL-6, IL-10).
  • SARS-CoV-2 antibody titers were also determined.

Main Results:

  • Vaccinated subjects showed reduced prothrombin and fibrinogen levels but higher prevalence outside normal ranges for prothrombin and antithrombin.
  • Thrombin generation analysis revealed a more procoagulant profile with shortened lag time and time-to-peak, and increased peak height.
  • Increased Von Willebrand Factor (VWF) and active VWF indicated endothelial activation, alongside significantly elevated IL-10 levels.

Conclusions:

  • ChAdOx1-S vaccination is associated with significant alterations in hemostasis, including a procoagulant profile and endothelial activation.
  • Persistently increased IL-10 suggests ongoing immune system activity post-vaccination, potentially contributing to thrombotic risk.
  • These findings highlight a possible pathophysiological mechanism for increased thrombotic events after ChAdOx1-S vaccination.