A polyphenol-assisted IL-10 mRNA delivery system for ulcerative colitis

Area of Science:

• Biomedical Engineering
• Nanotechnology
• Immunology

Background:

• Modified messenger RNA (mRNA) shows therapeutic potential but faces delivery challenges due to enzymatic degradation.
• Efficient intracellular delivery is crucial for mRNA-based therapies, particularly for treating inflammatory diseases.

Purpose of the Study:

• To develop a polyphenol-assisted delivery system for efficient in vivo delivery of IL-10 mRNA.
• To evaluate the therapeutic efficacy of the designed nanostructure in murine models of colitis.

Main Methods:

• A layer-by-layer nanostructure was created by complexing IL-10 mRNA with ellagic acid, polyetherimide, and bilirubin-modified hyaluronic acid.
• The nanostructure's efficacy was assessed in dextran sulfate sodium salt-induced acute and chronic murine colitis models.

Main Results:

• The nanostructure successfully delivered IL-10 mRNA, up-regulating IL-10 levels and inhibiting inflammatory factors.
• The system demonstrated therapeutic efficacy in colitis models, promoting mucosal repair and protecting colonic cells.
• No systemic toxicity was observed with the designed delivery system.

Conclusions:

• The polyphenol-assisted nanostructure provides an effective and safe platform for mRNA delivery.
• This strategy holds promise for the development of novel treatments for ulcerative colitis.