Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Sep 1, 2025

Measuring Skeletal Muscle Thermogenesis in Mice and Rats

Measuring Skeletal Muscle Thermogenesis in Mice and Rats

Published on: July 27, 2022

Measuring Skeletal Muscle Thermogenesis in Mice and Rats.

Christina A Watts¹, Alexandra Haupt², Jordan Smith³

¹School of Biomedical Sciences, Kent State University; cwatts9@kent.edu.

Journal of Visualized Experiments : Jove

|August 15, 2022

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

In a room or on Zoom? Trying out an online research course for people with intellectual disabilities.

Journal of intellectual disabilities : JOID·2026

Same author

Impact of timing of nontuberculous mycobacterial pulmonary disease antibiotic initiation on hospitalizations in Japan.

Respiratory investigation·2026

Same author

Patient awareness and perspectives regarding the value of focal therapy for localized prostate cancer: A cross-sectional study.

Urologic oncology·2026

Same author

Behavioral Risk Factors and Seroprevalence for Human Papillomavirus in Sinonasal Carcinoma: A Multi-Institutional Case-Control Study.

Head & neck·2026

Same author

Convenience Versus Continuity: A Pilot Study of Care Transfer to a Satellite Clinic for Patients With Plasma Cell Dyscrasias.

Cancer control : journal of the Moffitt Cancer Center·2025

Same author

Cross-cultural adaptation and validation of a Resistance Training Skill Battery for use in Chinese-speaking adolescents.

PeerJ·2025

Researchers developed a new method to directly measure skeletal muscle temperature in rodents. This technique reveals how stimuli like predator odor and oxytocin influence muscle thermogenesis and energy expenditure.

Area of Science:

Physiology
Metabolic Homeostasis
Thermogenesis Research

Background:

Understanding metabolic homeostasis and energy expenditure is crucial.
Direct links between neural, myocellular, and molecular thermogenesis mechanisms and measurable muscle temperature changes are lacking.

Purpose of the Study:

To describe a novel method for direct measurement of skeletal muscle temperature in mice and rats.
To demonstrate the method's utility in studying thermogenesis control mechanisms.

Main Methods:

Surgically implanting remote temperature transponders within the skeletal muscle of mice and rats.
Habituating animals to the testing environment and procedure.
Measuring muscle temperature changes in response to pharmacological (oxytocin) or contextual (predator odor) stimuli, and during treadmill exercise.

More Related Videos

Determining Basal Energy Expenditure and the Capacity of Thermogenic Adipocytes to Expend Energy in Obese Mice

Determining Basal Energy Expenditure and the Capacity of Thermogenic Adipocytes to Expend Energy in Obese Mice

Published on: November 11, 2021

Infrared Thermography for the Detection of Changes in Brown Adipose Tissue Activity

Infrared Thermography for the Detection of Changes in Brown Adipose Tissue Activity

Published on: September 28, 2022

Related Experiment Videos

Last Updated: Sep 1, 2025

Measuring Skeletal Muscle Thermogenesis in Mice and Rats

Measuring Skeletal Muscle Thermogenesis in Mice and Rats

Published on: July 27, 2022

Determining Basal Energy Expenditure and the Capacity of Thermogenic Adipocytes to Expend Energy in Obese Mice

Determining Basal Energy Expenditure and the Capacity of Thermogenic Adipocytes to Expend Energy in Obese Mice

Published on: November 11, 2021

Infrared Thermography for the Detection of Changes in Brown Adipose Tissue Activity

Infrared Thermography for the Detection of Changes in Brown Adipose Tissue Activity

Published on: September 28, 2022

Main Results:

Successfully demonstrated the detection of rapid changes in skeletal muscle temperature.
Predator odor (contextual stimulus) was shown to induce muscle thermogenesis.
Oxytocin (pharmacological stimulus) was shown to suppress muscle temperature.

Conclusions:

The described method provides direct measurements of skeletal muscle temperature, advancing thermogenesis research.
This technique effectively elucidates neural, sympathetic, and skeletal muscle mechanisms controlling thermogenesis.
The findings highlight the method's efficacy in detecting stimulus-induced muscle temperature variations.