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Updated: Jul 21, 2026

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

Published on: March 9, 2021

Temperature-responsive Pickering high internal phase emulsions for recyclable efficient interfacial biocatalysis.

Chao Wang^1,2, Hui Chi¹, Fan Zhang^1,2

¹Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences Changchun 130022 PR China byungang@ciac.ac.cn xukun@ciac.ac.cn.

Chemical Science

|August 17, 2022

Summary

Thermoresponsive Pickering high internal phase emulsions (HIPEs) offer a novel solution for efficient biocatalysis. This system enables easy enzyme separation and reuse, enhancing catalytic efficiency for green chemical processes.

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Area of Science:

Biocatalysis and green chemistry
Materials science and nanotechnology

Background:

Biocatalysis is crucial for sustainable chemical production, but challenges remain in enzyme recovery and process efficiency.
Current methods often lack integrated solutions for separation, reuse, and high catalytic activity.

Purpose of the Study:

To develop controllable scaffolds for efficient biocatalysis using thermoresponsive Pickering high internal phase emulsions (HIPEs).
To address the limitations of product separation, enzyme recovery, and catalytic efficiency in biocatalytic processes.

Main Methods:

Fabrication of ultra-high-surface-area Pickering HIPEs stabilized by lipase B immobilized on modified starch particles.
Tuning the thermoresponsive properties by adjusting the degree of substitution of modified starches.

Main Results:

The developed HIPEs exhibit temperature-dependent emulsification and demulsification, facilitating enzyme separation and reuse.
Immobilized lipase demonstrated significantly improved catalytic efficiency within the HIPE system.
The switching temperature of the HIPEs could be precisely controlled for enzyme compatibility.

Conclusions:

Thermoresponsive Pickering HIPEs provide a versatile and controllable platform for efficient interfacial biocatalysis.
This system simplifies enzyme recovery and reuse, paving the way for greener industrial chemical processes.