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Updated: Aug 10, 2026
06:25
Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Screening of amphetamines by gradient microbore liquid chromatography and pre-column technology
Journal of Chromatography
|April 22, 1987
Summary
This study presents a new method for detecting amphetamine-type drugs in urine using on-line pre-concentration and high-performance liquid chromatography. The technique efficiently screens for these substances, aiding in doping control.
Area of Science:
- Analytical Chemistry
- Forensic Science
- Biochemistry
Background:
- Amphetamine-type drugs (ATDs) are widely abused and frequently screened for in biological samples.
- Existing methods for ATD detection in urine can be complex and time-consuming.
- A need exists for rapid, automated, and sensitive screening methods for ATDs.
Purpose of the Study:
- To develop and validate an on-line chromatographic method for screening underivatized amphetamines in human and horse urine.
- To assess the method's suitability for automated analysis and doping control.
Main Methods:
- Utilized on-line pre-concentration with sequential hydrophobic and cation-exchange sorbent columns.
- Employed gradient microbore high-performance liquid chromatography (HPLC).
- Detected underivatized amphetamines using UV detection at 210 nm.
Main Results:
- Successfully screened amphetamine-type drugs across a wide polarity range in both human and horse urine.
- Demonstrated the capability for automated liquid chromatographic screening.
- Identified underivatized amphetamines effectively via UV detection.
Conclusions:
- The developed method offers a robust and potentially automated approach for amphetamine screening in urine.
- This technique shows significant promise for applications in doping control and forensic toxicology.
- The use of on-line pre-concentration enhances efficiency for detecting a broad spectrum of amphetamines.
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