Transcriptome Analysis of Dendrobine Biosynthesis in Trichoderma longibrachiatum MD33

Area of Science:

• Biotechnology
• Molecular Biology
• Metabolic Engineering

Background:

• Dendrobine, a key compound from Dendrobium nobile, possesses significant pharmacological properties.
• Trichoderma longibrachiatum MD33, isolated from D. nobile, is capable of producing dendrobine.
• Understanding the regulation of dendrobine biosynthesis is crucial for its biotechnological production.

Purpose of the Study:

• To investigate the effect of Methyl Jasmonate (MeJA) on dendrobine production in T. longibrachiatum MD33.
• To elucidate the molecular mechanisms underlying MeJA-induced dendrobine accumulation using transcriptome analysis.

Main Methods:

• Trichoderma longibrachiatum MD33 was treated with MeJA (20 μmol/L).
• RNA sequencing technology was employed for transcriptome analysis of MeJA-treated and control groups.
• Differentially expressed genes (DEGs) were identified and annotated using Kyoto Encyclopedia of Genes and Genomes (KEGG).

Main Results:

• MeJA treatment increased dendrobine production by 44.6% compared to the control.
• A total of 444 DEGs were identified, including 226 up-regulated and 218 down-regulated genes.
• DEGs were associated with alkaloid biosynthesis, MVA pathway, P450, aminotransferase, methyltransferase, and transcription factor families, indicating MeJA's regulatory role.

Conclusions:

• MeJA significantly enhances dendrobine production in T. longibrachiatum MD33.
• Dendrobine biosynthesis likely involves the MVA pathway and is regulated by a complex network of genes, including transcription factors.
• The study identifies key genes and pathways involved in MeJA-induced dendrobine accumulation, providing targets for further research and metabolic engineering.