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Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

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Published on: June 30, 2018

Morpholine-Functionalized Multicomponent Metallacage as a Vector for Lysosome-Targeted Cell Imaging.

Qian Feng^1,2,3, Tianfeng Yang², Lingzhi Ma^1,4

¹State Key Laboratory for Mechanical Behavior of Materials, Shaanxi International Research Center for Soft Matter, School of Materials Science and Engineering, Xi'an Jiaotong University, Xi'an 710049, P. R. China.

ACS Applied Materials & Interfaces

|August 18, 2022

Summary

We developed a morpholine-functionalized metallacage for targeted cell imaging. This delivery vector efficiently carries dyes to lysosomes, maintaining their fluorescence for enhanced biological applications.

Keywords:

cell imaging fluorescence host−guest complexation metallacages targeted delivery

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Area of Science:

Coordination Chemistry
Supramolecular Chemistry
Biomedical Engineering
Cellular Imaging

Background:

Metallacages are promising delivery vectors for biological systems due to their defined cavities and functionalities.
Effective delivery of imaging agents is crucial for accurate cellular diagnostics.
Aggregation of dyes in aqueous solutions often quenches their fluorescence, limiting their utility.

Purpose of the Study:

To develop a morpholine-functionalized metallacage for lysosome-targeted cell imaging.
To investigate the metallacage's ability to prevent dye aggregation and maintain fluorescence.
To demonstrate the metallacage's utility as a vector for delivering multiple colored dyes for targeted imaging.

Main Methods:

Synthesis of a morpholine-functionalized metallacage.
Host-guest complexation studies with various dyes.
Measurement of fluorescence quantum yield and aggregation behavior.
In vitro cell imaging experiments targeting lysosomes.

Main Results:

Efficient host-guest interactions within the metallacage prevented dye aggregation in aqueous solutions.
The fluorescence quantum yield of the host-guest complexes reached 74.40%.
The metallacage successfully delivered blue, green, and red emitting dyes into lysosomes for targeted imaging.

Conclusions:

The morpholine-functionalized metallacage serves as an effective vector for delivering functional dyes.
This system enables robust, targeted lysosome imaging with preserved dye fluorescence.
The developed metallacage platform expands the application scope of metallacages in biomedical engineering.