Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transforming the cytokine literature into a resource for experimental analysis and discovery.

bioRxiv : the preprint server for biology·2026
Same author

Author Correction: Random access and semantic search in DNA data storage enabled by Cas9 and machine-guided design.

Nature communications·2026
Same author

A single-cell cytokine dictionary of human peripheral blood.

bioRxiv : the preprint server for biology·2025
Same author

Massively parallel assay of human splice variants reveals cis-regulatory drivers of disease-associated and cell type-specific splicing regulation.

bioRxiv : the preprint server for biology·2025
Same author

Design of overlapping genes using deep generative models of protein sequences.

bioRxiv : the preprint server for biology·2025
Same author

Random access and semantic search in DNA data storage enabled by Cas9 and machine-guided design.

Nature communications·2025
Same journal

Sub1 contributes to heart failure with preserved ejection fraction driven by aging in mice.

Nature communications·2026
Same journal

The BRCA1-A complex restricts replication fork reversal-dependent DNA repair in ATM deficient cells.

Nature communications·2026
Same journal

Signaling downstream of tumor-stroma interaction regulates mucinous colorectal adenocarcinoma apicobasal polarity.

Nature communications·2026
Same journal

Click-polymerized polyenamine membranes for efficient lithium extraction.

Nature communications·2026
Same journal

Joint trajectories of brain atrophy, white matter hyperintensities and cognition quantify brain maintenance.

Nature communications·2026
Same journal

Proton shuttling at electrochemical interfaces under alkaline hydrogen evolution.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Aug 31, 2025

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.3K

A nanopore interface for higher bandwidth DNA computing.

Karen Zhang1, Yuan-Jyue Chen2, Delaney Wilde1

  • 1Paul G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA.

Nature Communications
|August 20, 2022
PubMed
Summary
This summary is machine-generated.

This study introduces a new DNA strand displacement (DSD) circuit readout method using nanopore sequencing. This technique significantly enhances the parallel detection of DSD circuit outputs, advancing nanoscale information processing.

More Related Videos

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

13.6K
Nanopore DNA Sequencing for Metagenomic Soil Analysis
07:33

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

30.8K

Related Experiment Videos

Last Updated: Aug 31, 2025

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.3K
Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

13.6K
Nanopore DNA Sequencing for Metagenomic Soil Analysis
07:33

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

30.8K

Area of Science:

  • Nanotechnology
  • Molecular Computing
  • Biotechnology

Background:

  • DNA strand displacement (DSD) is a key primitive for nanoscale information processing, with applications in diagnostics and molecular computing.
  • Current DSD circuit output detection relies on fluorescence spectroscopy, which has limited parallel detection capabilities due to spectral overlap of reporters.
  • Scalability of DSD circuits is hindered by the low output bandwidth of fluorescence-based detection methods.

Purpose of the Study:

  • To develop a novel, multiplexable, and sequencing-free readout method for DNA strand displacement (DSD) circuits.
  • To overcome the limitations of fluorescence spectroscopy in detecting parallel outputs from DSD circuits.
  • To enable real-time, kinetic measurement of DSD circuit activity with enhanced output bandwidth.

Main Methods:

  • Utilized nanopore sensor array technology (Oxford Nanopore Technologies' MinION device) for direct detection of barcoded output strands.
  • Developed a multiplexable readout strategy enabling high-throughput, sequencing-free measurement.
  • Applied the method for real-time kinetic measurement of DSD circuit activity.

Main Results:

  • Demonstrated a sequencing-free readout method for DSD circuit activity.
  • Achieved highly parallel, direct detection of barcoded output strands using nanopore technology.
  • Increased the output bandwidth of DSD circuits by an order of magnitude compared to fluorescence spectroscopy.

Conclusions:

  • The developed nanopore-based readout method significantly enhances the output bandwidth of DSD circuits.
  • This approach offers a scalable solution for multiplexed detection, advancing the field of molecular computing.
  • The technology enables real-time kinetic measurements, paving the way for more sophisticated DNA-based information processing systems.