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Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
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Endoplasmic reticulum membrane protein MoScs2 is important for asexual development and pathogenesis of Magnaporthe

Jun Zhang1, Xuehang Chen1, Zifeng Yang1

  • 1State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China.

Frontiers in Microbiology
|August 22, 2022
PubMed

Insights

The MoSCS2 gene is crucial for the growth and development of the rice blast fungus Magnaporthe oryzae. Its absence impairs fungal growth, spore formation, and infection capabilities.

Area of Science:

  • Plant Pathology
  • Mycology
  • Molecular Biology

Background:

  • Secretory proteins undergo folding and modification in the endoplasmic reticulum (ER).
  • Scs2 protein absence in yeast causes ER dysfunction, but its role in filamentous fungi like Magnaporthe oryzae is unknown.
  • Understanding ER-associated proteins is vital for controlling fungal pathogens.

Purpose of the Study:

  • To identify and characterize the MoSCS2 gene in Magnaporthe oryzae.
  • To investigate the function of MoSCS2 in fungal growth, development, and pathogenicity.
  • To elucidate MoSCS2's role in ER stress response.

Main Methods:

  • Subcellular localization of MoSCS2 protein.
  • Construction and analysis of MoSCS2 deletion mutant (ΔMoscs2).
  • Phenotypic analysis of growth, conidiation, and conidial morphology.
  • Assessment of appressorium formation and function.
  • Pathogenicity assays on host plants.

Main Results:

  • MoSCS2 localizes to the endoplasmic reticulum.
  • ΔMoscs2 mutant exhibits reduced growth, conidiation, and abnormal conidial morphology.
  • MoSCS2 deficiency delays appressorium formation and impairs host penetration.
  • Pathogenicity is significantly reduced in the ΔMoscs2 mutant.
  • MoSCS2 is involved in cell wall integrity and ER stress response.

Conclusions:

  • MoSCS2 is essential for Magnaporthe oryzae growth, asexual reproduction, and conidia development.
  • MoSCS2 plays a critical role in infection-related morphogenesis and pathogenicity.
  • MoSCS2 is involved in regulating cell wall integrity and ER stress response in M. oryzae.
Keywords:
ER-PMMagnaporthe oryzaeasexual developmentpathogenesistail-anchored protein

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