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Updated: Aug 31, 2025

Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane SSM-Based Electrophysiology
Published on: May 3, 2021
Amidosquaramides - a new anion binding motif with pH sensitive anion transport properties
Luke A Marchetti1, Tobias Krämer1, Robert B P Elmes1,2
1Department of Chemistry, National University of Ireland Maynooth, Maynooth, Ireland. robert.elmes@mu.ie.
Abstract:
Stimuli responsive anion transport is becoming an important aspect of supramolecular anion recognition chemistry. Herein, we report the synthesis of a family of anion receptors that incorporate a new anion binding motif, amidosquaramides. We show using experimental and computational methods that these receptors have pKa values close to physiological pH but also display intramolecular H-bonding interactions that affect anion recognition. Moreover, moderate activity in a Cl-/NO3- exchange assay is observed at physiological pH that can be effectively 'switched on' when repeated under acidic conditions. The reported findings provide synthetic methods that can be used for the construction of more complex squaramide based anion receptors and also provide insight into the importance of conformational analysis when considering receptor design.
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