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Updated: Aug 31, 2025

Induction of Ocular Surface Inflammation and Collection of Involved Tissues
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Induction of Ocular Surface Inflammation and Collection of Involved Tissues

Published on: August 4, 2022

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Induction of Ocular Surface Inflammation and Collection of Involved Tissues

Jeeshan Singh1, Xiaomei Shan1, Aparna Mahajan1

  • 1Department of Internal Medicine 3-Rheumatology and Immunology, Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen; Deutsches Zentrum für Immuntherapie, Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen.

Journal of Visualized Experiments : Jove
|August 22, 2022
PubMed

Insights

Ocular surface inflammation involves neutrophils forming aggregated neutrophil extracellular traps (aggNETs) that block meibomian glands (MG). This study details a method to induce and study MG dysfunction for preclinical anti-inflammatory research.

Area of Science:

  • Ophthalmology
  • Immunology
  • Histopathology

Background:

  • Ocular surface diseases affect the cornea, conjunctiva, and ocular glands.
  • Meibomian glands (MG) are crucial for tear film stability.
  • Neutrophil extracellular traps (NETs) are implicated in ocular surface inflammation and MG dysfunction.

Purpose of the Study:

  • To present a method for inducing ocular surface inflammation and meibomian gland dysfunction.
  • To describe procedures for collecting and processing ocular tissues.
  • To enable preclinical investigation of anti-inflammatory interventions.

Main Methods:

  • Induction of ocular surface inflammation and MG dysfunction in a mouse model.
  • Detailed protocols for collecting cornea, conjunctiva, and eyelids.
  • Morphological and histopathological analysis of ocular tissues and exudates.

Main Results:

  • Aggregated neutrophil extracellular traps (aggNETs) were observed to occlude MG outlets.
  • Morphological and histopathological features of MG dysfunction were characterized.
  • Ocular exudates were utilized to assess the inflammatory state.

Conclusions:

  • The presented method effectively induces ocular surface inflammation and MG dysfunction.
  • This model allows for detailed study of the mechanisms underlying MG dysfunction.
  • It provides a platform for evaluating preclinical anti-inflammatory treatments for ocular surface diseases.

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