Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Deconvolution01:20

Deconvolution

240
Deconvolution, also known as inverse filtering, is the process of extracting the impulse response from known input and output signals. This technique is vital in scenarios where the system's characteristics are unknown, and they must be inferred from the observable signals.
Deconvolution involves several mathematical techniques to derive the impulse response. One common approach is polynomial division. In this method, the input and output sequences are treated as coefficients of...
240
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.5K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.5K
Computed Tomography01:10

Computed Tomography

5.6K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
5.6K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

5.1K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
5.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TRAF6 Lactylation in Glycolytic Macrophages Drives NF-κB Signaling and M1 Polarization During Orthodontic Tooth Movement.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Double Zernike polynomial-based desensitization design method for off-axis optical systems.

Optics express·2026
Same author

CRISPR-based next-generation molecular diagnostics for bone infection.

Frontiers in cell and developmental biology·2026
Same author

White cell - platelet ratio: A strong indicator for early mortality in liver cirrhosis patients with esophagogastric varices.

Scientific reports·2026
Same author

Fast-Scan Voltammetry-Driven Nanoprobes for the Intracellular PTP1B Activity Assay and Invasiveness Evaluation of Single Tumor Cells.

Analytical chemistry·2026
Same author

Optical metasurfaces for general vision processing on the edge.

Nature·2026

Related Experiment Video

Updated: Aug 31, 2025

Author Spotlight: Enhancement of Salient Object Detection for Smart Grid Applications
03:31

Author Spotlight: Enhancement of Salient Object Detection for Smart Grid Applications

Published on: December 15, 2023

621

Boosting Photon-Efficient Image Reconstruction With A Unified Deep Neural Network.

Jiayong Peng, Zhiwei Xiong, Hao Tan

    IEEE Transactions on Pattern Analysis and Machine Intelligence
    |August 22, 2022
    PubMed
    Summary

    This study introduces a deep neural network for photon-efficient imaging, overcoming low signal-to-background ratio (SBR) and multiple returns. The method reconstructs high-fidelity depth and intensity images, even in challenging low-light conditions.

    More Related Videos

    Author Spotlight: Integrated Photoacoustic, Ultrasound, and Angiographic Tomography (PAUSAT) for NonInvasive Whole-Brain Imaging of Ischemic Stroke
    06:45

    Author Spotlight: Integrated Photoacoustic, Ultrasound, and Angiographic Tomography (PAUSAT) for NonInvasive Whole-Brain Imaging of Ischemic Stroke

    Published on: June 2, 2023

    1.7K
    Deep Learning-Based Segmentation of Cryo-Electron Tomograms
    10:25

    Deep Learning-Based Segmentation of Cryo-Electron Tomograms

    Published on: November 11, 2022

    9.3K

    Related Experiment Videos

    Last Updated: Aug 31, 2025

    Author Spotlight: Enhancement of Salient Object Detection for Smart Grid Applications
    03:31

    Author Spotlight: Enhancement of Salient Object Detection for Smart Grid Applications

    Published on: December 15, 2023

    621
    Author Spotlight: Integrated Photoacoustic, Ultrasound, and Angiographic Tomography (PAUSAT) for NonInvasive Whole-Brain Imaging of Ischemic Stroke
    06:45

    Author Spotlight: Integrated Photoacoustic, Ultrasound, and Angiographic Tomography (PAUSAT) for NonInvasive Whole-Brain Imaging of Ischemic Stroke

    Published on: June 2, 2023

    1.7K
    Deep Learning-Based Segmentation of Cryo-Electron Tomograms
    10:25

    Deep Learning-Based Segmentation of Cryo-Electron Tomograms

    Published on: November 11, 2022

    9.3K

    Area of Science:

    • Computational imaging
    • Computer vision
    • Deep learning for image reconstruction

    Background:

    • Photon-efficient imaging utilizes single-photon sensors for 3D image capture, but faces challenges from low photon counts, low signal-to-background ratio (SBR), and multiple returns.
    • These limitations hinder reconstruction performance in applications requiring high fidelity under low optical flux.

    Purpose of the Study:

    • To develop a unified deep neural network capable of simultaneously recovering depth maps and intensity images from photon-efficient measurements.
    • To explicitly address the challenges of low SBR and multiple returns in photon-efficient imaging.

    Main Methods:

    • A novel deep neural network architecture featuring an encoder with a non-local block for spatial-temporal correlation exploitation and two decoders for depth and intensity recovery.
    • Integration of a noise prior block informed by background noise photon statistics to enhance reconstruction.
    • Training on simulated data with validation on real-world imaging systems.

    Main Results:

    • The proposed network achieves superior reconstruction fidelity for depth and intensity images, even with extremely low photon counts, low SBR, and significant blur from multiple returns.
    • Performance significantly surpasses existing methods in challenging imaging scenarios.
    • The network demonstrates strong generalization capabilities from simulated to real-world data.

    Conclusions:

    • The unified deep neural network effectively overcomes key limitations in photon-efficient imaging, enabling high-quality 3D reconstruction.
    • This approach broadens the applicability of photon-efficient imaging in scenarios with strict optical flux constraints.
    • The method offers a significant advancement for applications requiring precise imaging under low-light conditions.