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Related Experiment Video

Updated: Aug 31, 2025

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

Multiplexed Single-Cell in Situ Protein Profiling.

Yi Chen¹, Jia Guo¹

¹Biodesign Institute & School of Molecular Sciences, Arizona State University, Tempe, Arizona 85287, United States.

ACS Measurement Science Au

|August 23, 2022

Summary

This summary is machine-generated.

Multiplexed protein imaging enables single-cell analysis in native locations, revealing cell heterogeneity crucial for understanding physiology and disease. This technology overcomes limitations of bulk methods and low-plex assays for advanced biological and medical insights.

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Last Updated: Aug 31, 2025

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

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Area of Science:

Cellular Biology
Biomedical Sciences
Proteomics

Background:

Bulk protein quantification obscures cellular heterogeneity in complex systems.
Conventional immunofluorescence and immunohistochemistry have limited multiplexing capabilities.
Understanding cell physiology and disease pathogenesis requires in situ protein profiling.

Purpose of the Study:

To review recent advances in multiplexed single-cell in situ protein profiling technologies.
To discuss the advantages and limitations of these cutting-edge methods.
To highlight their applications and challenges in biology and medicine.

Main Methods:

Technological advancements in multiplexed protein imaging.
In situ analysis of distinct proteins within individual cells.
Single-cell resolution of protein expression in native cellular environments.

Main Results:

Multiplexed imaging allows simultaneous analysis of numerous proteins in single cells.
These methods provide deeper insights into cell type classification and signaling networks.
Applications span tissue architecture, disease diagnosis, prognosis, and treatment monitoring.

Conclusions:

Multiplexed single-cell protein profiling is a transformative technology for biological and biomedical research.
It addresses limitations of traditional methods, offering high-resolution insights.
Future solutions are needed to overcome current challenges and maximize potential.