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Separation methods for system-wide profiling of protein terminome
Rui Wang1, Zhongjie Wang1, Haojie Lu1,2
1Shanghai Cancer Center and Institutes of Biomedical Sciences, Fudan University, Shanghai, People's Republic of China.
Proteomics
|August 23, 2022
Summary
Terminomics studies protein N- and C-termini, crucial for biological functions. This review covers advanced enrichment methods for high-throughput analysis, aiding biomarker and therapeutic target discovery.
Area of Science:
- Biochemistry
- Proteomics
- Molecular Biology
Background:
- Protein N- and C-termini possess unique biochemical properties and functions.
- These termini are critical for protein stability, localization, and biological processes.
- Post-translational modifications and proteolytic cleavage generate diverse proteoforms at protein termini.
Purpose of the Study:
- To review recent advancements in protein N- and C-termini enrichment methods.
- To highlight the applications of these methods in various biological samples.
- To explore future directions for terminomics in identifying protease substrates and disease biomarkers.
Main Methods:
- High-throughput terminomics analysis strategies.
- Enrichment techniques for protein N-termini.
- Enrichment techniques for protein C-termini.
Main Results:
- Summary of current state-of-the-art terminomics enrichment strategies.
- Overview of the application scope of these methods across different sample types.
- Identification of key areas for future terminomics research.
Conclusions:
- Terminomics is a rapidly advancing field with significant potential.
- Advanced enrichment methods enable comprehensive analysis of protein termini.
- Future applications include protease substrate profiling and biomarker discovery for diseases and therapeutic targets.
