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A covalent organic framework nanosheet-based electrochemical aptasensor with sensitive detection performance.

Hong-Kai Li1, Ya-Xuan An1, En-Hui Zhang1

  • 1Tianjin Key Laboratory of Structure and Performance for Functional Molecules, College of Chemistry, Tianjin Normal University, Tianjin, 300387, PR China.

Analytica Chimica Acta
|August 23, 2022
PubMed
Summary

Covalent organic framework nanosheets (COF NS) offer enhanced biosensing capabilities. A novel Tp-Bpy COF NS material significantly improves the detection of ultra-trace tobramycin in electrochemical aptasensors.

Keywords:
AptamerCovalent organic framework nanosheetElectrochemical aptasensorElectrochemical impedance spectroscopyTobramycin

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Covalent organic framework nanosheets (COF NS) are gaining importance due to their unique properties like high permeability, ordered structure, and excellent dispersability.
  • These properties enable widespread applications in separation, catalysis, sensing, and optical devices.

Purpose of the Study:

  • To prepare and characterize a novel Tp-Bpy COF NS material.
  • To utilize the synthesized COF NS for immobilizing aptamers to create a sensitive electrochemical aptasensor.
  • To evaluate the performance of the COF NS-based aptasensor for detecting ultra-trace tobramycin.

Main Methods:

  • Interfacial synthesis of 2,4,6-triformylphloroglucinol (Tp) and 5,5'-diamino-2,2'-bipyridine (Bpy) to form Tp-Bpy COF NS.
  • Characterization of the COF NS for film morphology, surface area, pore size, stability, and functional sites.
  • Immobilization of aptamers onto the COF NS surface for electrochemical aptasensor construction.
  • Electrochemical detection of tobramycin using the developed aptasensor.

Main Results:

  • Successfully synthesized Tp-Bpy COF NS with desirable characteristics including film morphology, high surface area, large pores, and excellent stability.
  • The Tp-Bpy COF NS demonstrated versatile functional sites suitable for aptamer immobilization.
  • The developed electrochemical aptasensor based on Tp-Bpy COF NS exhibited significantly enhanced biosensing performance for ultra-trace tobramycin detection compared to bulk COF.
  • The COF NS material facilitated superior immobilization of aptamers, leading to improved sensitivity and efficiency.

Conclusions:

  • Tp-Bpy COF NS is a promising functional material for constructing highly sensitive electrochemical aptasensors.
  • The use of COF NS significantly enhances biosensing performance, particularly for ultra-trace analyte detection.
  • This research contributes to the advancement of COF NS applications in electrochemical biosensing.