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Updated: Aug 31, 2025
Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
Published on: April 14, 2015
Dual amplification of a bio-barcode and auto-cycling primer extension for highly sensitive detection of miRNA
Nuli Xie1, Huimin Wang1, Jing Li2
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha, China. jinhuang@hnu.edu.cn.
Abstract:
MicroRNAs (miRNAs) can be used as biomarkers for the diagnosis and therapy of cancers. However, their low abundance and the complex environment in biological samples hinder miRNA detection. A dual amplification strategy based on the bio-barcode technique (BCA) and auto-cycling primer extension (APE) is proposed to detect miRNA targets in complex biological samples. The strategy shows a good sensitivity for miRNA-19a with a detection limit of 50 fM, and can effectively distinguish other similar miRNAs. It provides a new idea to combine nanoparticle-based amplification with nucleic acid-based amplification together for the sensitive detection of nucleic acid targets.
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