Metabolism of plasma fibronectin in rabbits with experimental hyperthyroidism and hypothyroidism

We previously reported that the concentration of plasma fibronectin (pFN) is increased in patients with hyperthyroidism and decreased in those with hypothyroidism. In this work, labeled pFN was given intravenously to euthyroid, hyperthyroid, and hypothyroid rabbits to examine its metabolism in states of thyroid dysfunction. In euthyroid rabbits, the serum concentration of thyroxine (T4) was 2.94 +/- 0.59 micrograms/dL, and that of pFN was 24.2 +/- 1.2 mg/dL. With 125I-FN as tracer, the half life was estimated as 72.8 +/- 2.6 hours, the fractional catabolic rate (FCR) as 2.08 +/- 0.07%/h, the rate of synthesis (SR) as 3.84 +/- 0.20 mg/kg/day and j1/j2 (the ratio of the exchange coefficients between intravascular and extravascular compartments) of pFN as 0.295 +/- 0.051. With 3H-FN as tracer, these values were not significantly different. In hyperthyroid rabbits, obtained by treatment with I-thyroxine, the serum T4 concentration was increased to 5.73 +/- 1.58 micrograms/dL and the pFN concentration to 29.6 +/- 2.2 mg/dL. In these animals, the half life of pFN was shortened to 59.8 +/- 1.0 hour, the FCR was slightly increased to 3.07 +/- 0.52%/h and the SR was greatly increased to 7.13 +/- 1.17 mg/kg/d. In hypothyroid rabbits, obtained by treatment with methylthiouracil, the serum T4 and pFN levels were reduced to 1.50 +/- 0.36 micrograms/dL and to 20.7 +/- 1.7 mg/dL, respectively, the FCR and SR were also decreased to 1.39 +/- 0.04%/h and 2.26 +/- 0.14 mg/kg/d, respectively. The values of j1/j2 did not significantly change during this work.(ABSTRACT TRUNCATED AT 250 WORDS)