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MiR-202-3p determines embryo viability during mid-blastula transition.

Ruiqin Hu^1,2, Yanna Xu^1,2, Bingshe Han^1,2

¹International Joint Research Centre for Marine Biosciences (Ministry of Science and Technology), College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.

Frontiers in Cell and Developmental Biology

|August 25, 2022

Summary

A newly identified microRNA, miR-202-3p, is essential for embryonic development in zebrafish. This microRNA regulates key genes, ensuring proper cell proliferation and survival during the mid-blastula transition (MBT) for embryonic viability.

Keywords:

NF-κB apoptosis miR-202-3p mid-blastula transition zebrafish

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Published on: June 6, 2016

Area of Science:

Developmental Biology
Genetics
Molecular Biology

Background:

microRNAs (miRNAs) are critical regulators of gene expression in animal development.
The mid-blastula transition (MBT) is a key stage in zebrafish embryogenesis where zygotic gene expression begins.
Factors controlling cell proliferation during MBT remain largely uncharacterized.

Purpose of the Study:

To identify novel factors regulating embryonic fate during the mid-blastula transition (MBT) in zebrafish.
To elucidate the role of microRNA miR-202-3p in zebrafish embryonic development.
To investigate the regulatory network controlled by miR-202-3p.

Main Methods:

Utilized miR-202-3p antagomirs to inhibit miRNA function.
Generated miR-202 knockout zebrafish (miR-202-/-) to assess in vivo function.
Performed transcriptome analysis to identify dysregulated RNAs in miR-202-/- embryos.
Identified direct targets of miR-202-3p using bioinformatic and experimental approaches.
Assessed the developmental impact of modulating target genes nfkbiaa, perp, and mgll.

Main Results:

Inhibition or deletion of miR-202 resulted in developmental termination at the MBT stage.
miR-202 deficiency led to dysregulation of over 250 RNAs and massive apoptosis.
nfkbiaa, perp, and mgll were identified as direct targets of miR-202-3p.
Modulation of nfkbiaa, perp, or mgll individually caused developmental defects.
nfkbiaa and perp were found to be mutually regulatory.

Conclusions:

miR-202-3p is a crucial determinant of embryonic fate during zebrafish MBT.
miR-202-3p regulates embryonic viability by controlling a network of genes involved in cell proliferation and survival.
The identified regulatory network involving miR-202-3p, nfkbiaa, perp, and mgll is essential for successful embryogenesis.