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The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...

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Related Experiment Video

Updated: Jun 26, 2026

Patterning of Embryonic Stem Cells Using the Bio Flip Chip

Patterning of Embryonic Stem Cells Using the Bio Flip Chip

Published on: October 1, 2007

Multicellular Cell Seeding on a Chip: New Design and Optimization towards Commercialization.

Trieu Nguyen^1,2, Linh Ho¹, Sakib M Moinuddin^1,2

¹Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, California Northstate University, Elk Grove, CA 95757, USA.

|August 25, 2022

Summary

This study optimizes microfluidic chip design for 3D multicellular co-cultures using circular micro-posts. This innovation enables rapid, cost-efficient fabrication of tissue-on-chip models for disease research and commercialization.

Keywords:

cell seeding clean room endothelial cells micro-milling microfluidics pulmonary arterial hypertension (PAH)smooth muscle cells

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Last Updated: Jun 26, 2026

Patterning of Embryonic Stem Cells Using the Bio Flip Chip

Patterning of Embryonic Stem Cells Using the Bio Flip Chip

Published on: October 1, 2007

Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors

Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors

Published on: May 21, 2008

Microfabrication of Chip-sized Scaffolds for Three-dimensional Cell cultivation

Microfabrication of Chip-sized Scaffolds for Three-dimensional Cell cultivation

Published on: May 12, 2008

Area of Science:

Biomedical Engineering
Microfluidics
Tissue Engineering

Background:

Tissue-on-chip models require optimized microfluidic platforms for disease research.
Microfluidic chips often use micro-posts to separate channels, but their design and fabrication are not optimized for scale.
Previous designs lack cost-efficient and rapid fabrication methods for commercialization.

Purpose of the Study:

To optimize microfluidic chip design for 3D multicellular co-culture applications.
To investigate the impact of micro-post structure and spacing on chip performance.
To develop a rapid and cost-efficient fabrication method for tissue-on-chip models.

Main Methods:

Experimental studies on a microfluidic platform.
In-depth theoretical analysis including COMSOL simulations and analytical solutions.
Computer-aided manufacturing (CAM) simulations for fabrication process evaluation.

Main Results:

Successful cell seeding achieved when pressure drops across posts exceed those across channel width.
Circular micro-posts demonstrated superior barrier effects, preventing channel cross-contamination.
CAM simulations confirmed circular-post fabrication is faster and simpler than hexagonal posts using micro-milling.

Conclusions:

Optimized microfluidic chip design using circular micro-posts enhances barrier function and cell seeding.
Circular micro-posts facilitate rapid, cost-efficient, large-scale fabrication via micro-milling and injection molding.
This approach advances commercialization of tissue-on-chip technology for disease modeling.