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Differences in Supragingival Microbiome in Patients with and without Full-Crown Prostheses
Manli Guo1, Zhidong Zhang2, Jiyuan Lu2
1Key Lab of Oral Diseases of Gansu Province, Northwest Minzu University, Lanzhou 730000, China.
Dentistry Journal
|August 25, 2022
Summary
Dental crowns alter oral microflora, increasing Hameophilus and cell motility while decreasing replication and translation. This research offers insights into the long-term oral safety of dental prostheses.
Area of Science:
- Microbiology
- Dental Science
- Bioinformatics
Background:
- Supragingival biofilm composition is crucial for oral health.
- Full-crown prostheses may influence the oral microbiome.
- Understanding these changes is vital for long-term dental material safety.
Purpose of the Study:
- To characterize the microflora profile of supragingival biofilm in patients with and without full-crown prostheses.
- To analyze the functional implications of microbial changes associated with dental crowns.
- To assess the impact of different crown materials (porcelain-fused-to-metal, all-ceramic) on oral microflora.
Main Methods:
- Collected plaque samples from patients with and without full-crown prostheses.
- Utilized 16S rRNA high-throughput sequencing for DNA sequencing.
- Employed Qiime, R, and PICRUSt2 for bioinformatics and functional analyses.
Main Results:
- Identified predominant phyla (Actinobacteria, Bacteroidetes, Firmicutes, Fusobacteria, Proteobacteria) and genera (Rothia, Porphyromonas, Prevotella, Streptococcus, Veillonella, Leptotrichia, Neisseria, Citrobacter, Pseudomonas).
- Observed a significant increase in the genus Hameophilus in patients with dental prostheses.
- Found increased cell motility and decreased replication/translation functions in biofilm from full-crown prostheses compared to natural teeth.
Conclusions:
- Full-crown prostheses significantly alter the oral microbial community structure and function.
- The genus Hameophilus and cell motility are key indicators of changes associated with dental prostheses.
- Findings provide insights into the safety of long-term use of materials in dental prosthetics.
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