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Raman Spectroscopy: Overview

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...

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Related Experiment Video

Updated: Aug 31, 2025

Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis

Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis

Published on: December 16, 2016

Alcohol Pretreatment to Eliminate the Interference of Micro Additive Particles in the Identification of Microplastics

Dunzhu Li^1,2, Emmet D Sheerin^1,3, Yunhong Shi^1,2

¹AMBER Research Centre and Centre for Research on Adaptive Nanostructures and Nanodevices (CRANN), Trinity College Dublin, Dublin D02 PN40, Ireland.

Environmental Science & Technology

|August 25, 2022

Summary

Raman spectroscopy can misidentify additive particles as microplastics. A new alcohol treatment protocol effectively distinguishes microplastics, revealing potential health risks from additive particle contamination.

Keywords:

Raman spectroscopy alcohol pretreatment hit quality index microadditive particles microplastic

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Last Updated: Aug 31, 2025

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Area of Science:

Environmental Science
Analytical Chemistry
Materials Science

Background:

Raman spectroscopy is crucial for analyzing microplastics (<20 μm).
Distinguishing microplastics from micro additive particles poses a significant challenge.
Additive particles can interfere with accurate microplastic identification.

Purpose of the Study:

To investigate the interference of slip additives in microplastic detection using Raman spectroscopy.
To develop and validate a new protocol for accurate microplastic identification.
To assess the release and potential health risks of micro additive particles.

Main Methods:

Characterization and comparison of Raman spectra of slip additives and polyethylene.
Development of an alcohol treatment protocol to eliminate additive particle interference.
Analysis of microplastic and additive particle release from common plastic products.

Main Results:

Slip additives showed high spectral similarity to microplastics (hit quality index 0.93-0.96).
The alcohol treatment protocol successfully eliminated additive particles, enabling accurate microplastic identification.
Common plastic products release both microplastics and micro additive particles, which can adsorb onto microplastics.

Conclusions:

Additive particles present a substantial interference in microplastic detection via Raman spectroscopy.
The developed alcohol treatment method offers a cost-effective solution for accurate microplastic analysis.
Micro additive particles pose potential health risks due to their release and adsorption onto microplastics.