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Updated: Aug 31, 2025
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Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
Published on: March 19, 2018
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Analysis of Gut Microbiome Using Fecal Samples.
Ahmed Gamal1, Mayyadah Alabdely2, Kelly Steller1
1Integrated Microbiome Core and Center for Medical Mycology, Department of Dermatology, Case Western Reserve University, Cleveland, OH, USA.
Methods in Molecular Biology (Clifton, N.J.)
|August 25, 2022
Summary
Accurate gut microbiome data is crucial for understanding its role in health and disease. This chapter details a reproducible stool sample analysis methodology to improve data consistency across studies.
Area of Science:
- Microbiology
- Genomics
- Bioinformatics
Background:
- The gut microbiome's role in disease and therapeutics is increasingly recognized.
- Accurate and reproducible microbial data is essential for in-depth health impact studies.
- Variability in DNA extraction and sequencing methods hinders cross-study result comparison.
Purpose of the Study:
- To present a detailed methodology for gut microbiome analysis.
- To standardize stool sample processing for improved data reproducibility.
- To facilitate reliable comparison of microbiome data across different research studies.
Main Methods:
- Detailed description of the microbiome analysis protocol.
- Focus on stool sample collection and processing.
- Emphasis on DNA extraction and sequencing techniques.
Main Results:
- Methodology ensures reproducible microbial data.
- Standardized protocol minimizes experimental variability.
- Data generated is comparable across studies.
Conclusions:
- Standardized microbiome analysis methodology is critical.
- Reproducible data enhances understanding of microbiome's health impact.
- This protocol supports robust microbiome research.
