Sediment Bacteria and Phosphorus Fraction Response, Notably to Titanium Dioxide Nanoparticle Exposure

Titanium dioxide nanoparticle (TiO₂ NP) toxicity to the growth of organisms has been gradually clarified; however, its effects on microorganism-mediated phosphorus turnover are poorly understood. To evaluate the influences of TiO₂ NPs on phosphorus fractionation and the bacterial community, aquatic microorganisms were exposed to different concentrations of TiO₂ NPs with different exposure times (i.e., 0, 10, and 30 days). We observed the adhesion of TiO₂ NPs to the cell surfaces of planktonic microbes by using SEM, EDS, and XRD techniques. The addition of TiO₂ NPs resulted in a decrease in the total phosphorus of water and an increase in the total phosphorus of sediments. Additionally, elevated TiO₂ NPs enhanced the sediment activities of reductases (i.e., dehydrogenase [0.19-2.25 μg/d/g] and catalase [1.06-2.92 μmol/d/g]), and significantly decreased the absolute abundances of phosphorus-cycling-related genes (i.e., gcd [1.78 × 10⁴-9.55 × 10⁵ copies/g], phoD [5.50 × 10³-5.49 × 10⁷ copies/g], pstS [4.17 × 10²-1.58 × 10⁶ copies/g]), and sediment bacterial diversity. TiO₂ NPs could noticeably affect the bacterial community, showing dramatic divergences in relative abundances (e.g., Actinobacteria, Acidobacteria, and Firmicutes), coexistence patterns, and functional redundancies (e.g., translation and transcription). Our results emphasized that the TiO₂ NP amount-rather than the exposure time-showed significant effects on phosphorus fractions, enzyme activity, phosphorus-cycling-related gene abundance, and bacterial diversity, whereas the exposure time exhibited a greater influence on the composition and function of the sediment bacterial community than the TiO₂ NP amount. Our findings clarify the responses of phosphorus fractions and the bacterial community to TiO₂ NP exposure in the water-sediment ecosystem and highlight potential environmental risks of the migration of untreated TiO₂ NPs to aquatic ecosystems.