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Oogenesis

Oogenesis

In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...

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Related Experiment Video

Updated: Aug 30, 2025

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Published on: October 11, 2015

Automated Denudation of Oocytes.

Rongan Zhai¹, Guanqiao Shan², Changsheng Dai²

¹School of Mechatronic Engineering and Automation, Shanghai University, Shanghai 200444, China.

|August 26, 2022

Summary

Automated robotic systems can now perform oocyte denudation for intracytoplasmic sperm injection (ICSI), improving yield and efficiency compared to manual methods. This technology enhances oocyte quality and fertilization rates, paving the way for standardized ICSI procedures.

Keywords:

ICSI oocyte denudation robotic system

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Last Updated: Aug 30, 2025

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Area of Science:

Reproductive Medicine
Robotics in Medicine
Biomedical Engineering

Background:

Manual oocyte denudation for intracytoplasmic sperm injection (ICSI) is labor-intensive, requiring extensive training and skilled operators.
Current manual methods face challenges with low yield rates and variable denudation efficiency due to human factors like fatigue and skill discrepancies.

Purpose of the Study:

To develop and evaluate a robotic system for automated oocyte denudation to overcome the limitations of manual procedures.
To enhance the yield rate and efficiency of oocyte denudation in ICSI.

Main Methods:

Development of a robotic system incorporating vision-based contact detection for precise micropipette positioning.
Implementation of algorithms for oocyte and cumulus cell recognition, automated calibration, and dynamic flow rate control during aspiration and deposition.
Experimental validation using mouse oocytes to assess system performance.

Main Results:

The robotic denudation system achieved a high yield rate of 97.0 ± 2.8% and denudation efficiency of 95.0 ± 0.8%.
Oocytes processed by the robotic system demonstrated fertilization rates and developmental competence comparable to those from manual denudation.

Conclusions:

The developed robotic system offers a viable solution for automated oocyte denudation, significantly improving upon manual techniques.
This advancement contributes to the automation and standardization of ICSI procedures, potentially improving outcomes in assisted reproductive technologies.