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miR-21 Targets Long Noncoding RNA PCAT29 to Promote Cell Proliferation in Neuroblastoma.

Zhong Jiang1, Xianjun Zhou1, Lulu Han2

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MicroRNA-21 (miR-21) promotes neuroblastoma (NB) cell proliferation by downregulating the tumor suppressor long noncoding RNA PCAT29. Restoring PCAT29 inhibits NB cell growth and promotes apoptosis.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNA (lncRNA) PCAT29 is a known tumor suppressor in various cancers.
  • The role of PCAT29 in neuroblastoma (NB) remains uncharacterized.

Purpose of the Study:

  • To investigate the function of PCAT29 in neuroblastoma.
  • To elucidate the relationship between PCAT29 and microRNA-21 (miR-21) in NB.

Main Methods:

  • Expression analysis of PCAT29 and miR-21 in NB tissues and cells using RT-qPCR.
  • Correlation analysis and RNA-RNA interaction prediction (IntaRNA 2.0) to assess the miR-21/PCAT29 interaction.
  • Functional assays including cell proliferation (propidium iodide, Ki67 staining) and apoptosis assays upon overexpression of miR-21 and PCAT29.

Main Results:

  • PCAT29 was downregulated, while miR-21 was upregulated in NB tissues compared to non-tumor tissues.
  • A significant inverse correlation was observed between PCAT29 and miR-21 expression.
  • miR-21 overexpression decreased PCAT29 levels, enhanced NB cell proliferation, and inhibited apoptosis. Conversely, PCAT29 overexpression suppressed proliferation and promoted apoptosis.

Conclusions:

  • MiR-21 targets PCAT29 in neuroblastoma, promoting cell proliferation and inhibiting apoptosis.
  • PCAT29 acts as a tumor suppressor in NB, potentially through its interaction with miR-21.