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Phase retardation and corneal sublayer thickness repeatability using ultrahigh-resolution polarization-sensitive OCT.

Rahul Patil1, Rohit Shetty, Yash Patel

  • 1From the Imaging, Biomechanics and Mathematical Modelling Solutions Lab, Narayana Nethralaya Foundation, Bangalore, India (Patil, Patel, Pisharody, Narasimhan, Sinha Roy); University Eye Clinic Maastricht, Maastricht University Medical Center, Maastricht, the Netherlands (Patil, Nuijts); Department of Cornea and Refractive Surgery, Narayana Nethralaya, Bangalore, India (Shetty); Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, Austria (Pircher, Hitzenberger).

Journal of Cataract and Refractive Surgery
|August 26, 2022
PubMed
Summary

Ultrahigh-resolution polarization-sensitive optical coherence tomography (PS-OCT) demonstrates excellent repeatability for measuring corneal birefringence and sublayer thickness. This advanced imaging offers superior clinical potential over existing OCT devices.

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Area of Science:

  • Ophthalmology
  • Biomedical Optics
  • Corneal Imaging

Background:

  • Corneal imaging relies on precise measurements of sublayer thickness and optical properties.
  • Existing optical coherence tomography (OCT) devices have limitations in assessing detailed corneal structures.
  • Polarization-sensitive OCT (PS-OCT) offers advanced capabilities for visualizing tissue birefringence.

Purpose of the Study:

  • To evaluate the repeatability of phase retardation and corneal sublayer thickness measurements using ultrahigh-resolution PS-OCT.
  • To compare the performance of PS-OCT with a high-resolution hybrid OCT (MS-39) for corneal imaging.

Main Methods:

  • Utilized a custom-built ultrahigh-resolution PS-OCT system and a commercial MS-39 OCT device.
  • Acquired three consecutive measurements per eye to assess repeatability.
  • Calculated phase retardation and corneal sublayer thicknesses from OCT data.
  • Assessed repeatability using the intraclass correlation coefficient (ICC).

Main Results:

  • Phase retardation en face maps revealed collagen fibril arrangement, with excellent repeatability (ICC >0.95).
  • Bowman layer and stromal layer thicknesses showed excellent repeatability (ICC >0.93 and >0.99, respectively).
  • PS-OCT demonstrated superior repeatability for epithelial thickness measurements compared to MS-39.

Conclusions:

  • Ultrahigh-resolution PS-OCT provides highly repeatable measurements of corneal birefringence and sublayer thicknesses.
  • PS-OCT shows advanced potential for routine clinical use in ophthalmology.
  • The combined assessment of corneal birefringence and thickness enhances diagnostic capabilities.