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Phase retardation and corneal sublayer thickness repeatability using ultrahigh-resolution polarization-sensitive OCT
Rahul Patil1, Rohit Shetty, Yash Patel
1From the Imaging, Biomechanics and Mathematical Modelling Solutions Lab, Narayana Nethralaya Foundation, Bangalore, India (Patil, Patel, Pisharody, Narasimhan, Sinha Roy); University Eye Clinic Maastricht, Maastricht University Medical Center, Maastricht, the Netherlands (Patil, Nuijts); Department of Cornea and Refractive Surgery, Narayana Nethralaya, Bangalore, India (Shetty); Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, Austria (Pircher, Hitzenberger).
Purpose:
To assess phase retardation and corneal sublayer thickness repeatability using ultrahigh-resolution polarization-sensitive optical coherence tomography (PS-OCT).
Setting:
Narayana Nethralaya Eye Hospital, Bangalore.
Design:
Observational.
Methods:
In this study, all eyes were imaged using a custom-built ultrahigh-resolution PS-OCT and high-resolution hybrid OCT (MS-39). The repeatability of phase retardation en face maps and corneal sublayer thickness profiles was evaluated. The reflectivity and phase retardation were calculated from the 2 orthogonal polarization channels to generate en face maps of phase retardation and corneal sublayer thicknesses. 3 consecutive measurements of all participants were acquired for each eye. For each measurement, the participant was asked to sit back and was realigned again. The repeatability was assessed using the intraclass correlation coefficient (ICC).
Results:
The study included 20 healthy eyes of 20 participants. The phase retardation en face maps showed preferential arrangement of collagen fibrils with least retardation in the apex and maximum retardation in the periphery. The phase retardation showed excellent repeatability (ICC >0.95) in all zones. The Bowman layer and stromal layer thicknesses were measured with excellent repeatability (ICC >0.93 and >0.99, respectively). Significant differences ( P < .05) in stromal layer thickness were observed between MS-39 and PS-OCT. The repeatability of epithelial thickness measurements was better with PS-OCT than MS-39.
Conclusions:
The combinational assessment of corneal birefringence and sublayer thicknesses shows the advanced potential of ultrahigh-resolution PS-OCT in routine clinical practice over current OCT devices.
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