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Updated: Aug 30, 2025
Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons
Published on: October 31, 2020
LuckyProfiler: an ImageJ plug-in capable of quantifying FWHM resolution easily and effectively for super-resolution
Mengting Li1, Qihang Song2, Yinghao Xiao2
1Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, China.
Abstract:
Quantifying the resolution of a super-resolution image is vital for biologists trying to apply super-resolution microscopy in various research fields. Among the reported image resolution estimation methods, the one that calculates the full width at half maximum (FWHM) of line profile, called FWHM resolution, continues the traditional resolution criteria and has been popularly used by many researchers. However, quantifying the FWHM resolution of a super-resolution image is a time-consuming, labor-intensive, and error-prone process because this method typically involves a manual and careful selection of one or several of the smallest structures. In this paper, we investigate the influencing factors in FWHM resolution quantification systematically and present an ImageJ plug-in called LuckyProfiler for biologists so that they can have an easy and effective way of quantifying the FWHM resolution of super-resolution images.
