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Updated: Aug 30, 2025

Development of a Nose-only Inhalation Toxicity Test Chamber That Provides Four Exposure Concentrations of Nano-sized Particles
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Performance Testing of a Venturi-Based Backpack Spray Decontamination System.

Alan Beswick1, Claire Bailey1, Brian Crook1

  • 1Health and Safety Executive, Buxton, Derbyshire, UK.

Applied Biosafety : Journal of the American Biological Safety Association
|August 29, 2022
PubMed
Summary
This summary is machine-generated.

Peracetic acid (PAA) demonstrated superior performance in a light decontamination system (LDS), achieving significant log reductions against bacterial spores. Hypochlorous acid (HOCl) showed less effectiveness, highlighting PAA

Keywords:
atomizationbiosecuritydecontaminationhypochlorous acidperacetic acidventuri lance

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Area of Science:

  • Microbiology
  • Biosecurity
  • Chemical Disinfection

Background:

  • Evaluating disinfectant efficacy is crucial for biosecurity.
  • Light Decontamination Systems (LDS) offer novel delivery methods for disinfectants.
  • Peracetic acid (PAA) and hypochlorous acid (HOCl) are common disinfectants with varying efficacies.

Purpose of the Study:

  • To assess the performance of peracetic acid (PAA) and hypochlorous acid (HOCl) using a Venturi-nozzle-based light decontamination system (LDS).
  • To evaluate the efficacy of these disinfectants against a range of challenging microorganisms, including bacterial spores.
  • To determine the suitability of the LDS for delivering disinfectants in biosecurity applications.

Main Methods:

  • Disinfectant chemicals (PAA and HOCl) were aerosolized using a Venturi-nozzle-based LDS with a handheld lance.
  • Tests included fixed and variable exposure times, multiple surface materials, and challenges with microorganisms like *Bacillus cereus* and *Bacillus anthracis* spores.
  • Live agent challenges were performed to simulate real-world decontamination scenarios.

Main Results:

  • Aerosolized PAA achieved 7- to 8-log reductions of all test challenges within 60 minutes.
  • PAA demonstrated extensive kill (≥6-log) on most surfaces and rapid spore kill against *B. anthracis* within 5 minutes.
  • HOCl was less effective, showing more variable results (4- to 6-log kill) and slower spore inactivation compared to PAA.

Conclusions:

  • Peracetic acid (PAA) is a highly effective disinfectant for rapid microbial kill, including spores, when delivered via an LDS.
  • The LDS successfully delivered both PAA and HOCl, indicating its potential for widespread biosecurity applications.
  • Disinfectant choice, microbial challenge, and placement significantly impact decontamination effectiveness.