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Related Experiment Video

Updated: Aug 30, 2025

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy
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Protocol for multi-scale light microscopy/electron microscopy neuronal imaging in mouse brain tissue.

Kenta Yamauchi1, Takahiro Furuta2, Shinichiro Okamoto3

  • 1Department of Neuroanatomy, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan; Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, Bunkyo-Ku, Tokyo 113-8421, Japan.

STAR Protocols
|August 29, 2022
PubMed
Summary

This study introduces a novel multi-scale imaging protocol combining light and electron microscopy for detailed neuronal structure analysis. The method enables visualization of both large neuronal processes and nanoscale features in mouse brains.

Keywords:
Cell BiologyMicroscopyNeuroscience

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Last Updated: Aug 30, 2025

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Area of Science:

  • Neuroscience
  • Microscopy techniques
  • Cell biology

Background:

  • Understanding neuronal structure across scales is crucial for neuroscience.
  • Existing imaging methods often lack the resolution or scope for comprehensive neuronal analysis.

Purpose of the Study:

  • To present a protocol for multi-scale imaging of neurons in mouse brain tissue.
  • To combine light microscopy (LM) and electron microscopy (EM) for detailed structural analysis.

Main Methods:

  • Protocol combines multi-scale light microscopy (LM) and electron microscopy (EM).
  • Utilizes EGFP-APEX2 fusion protein for dual LM/EM labeling.
  • Employs ScaleSF tissue clearing for enhanced imaging.
  • Involves detailed tissue slice preparation and successive imaging.

Main Results:

  • Successfully demonstrates a protocol for deciphering neuronal structural information across multiple spatial scales.
  • Enables visualization of neuronal processes at meter scale and specialized nanoscale structures.

Conclusions:

  • The presented multi-scale LM/EM protocol is effective for comprehensive neuronal structural analysis.
  • This technique advances the study of neuronal morphology in neuroscience research.