Related Experiment Video
Updated: Aug 30, 2025
Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
Proteomics analysis of coronary blood microparticles in patients with acute myocardial infarction
Yiping Ma1, Yujuan Yuan2, Zulipiya Aili1
1Xinjiang Emergency Center, People's Hospital of Xinjiang Uygur Autonomous Region, China.
Insights
Proteomics revealed differences in coronary microparticles between acute myocardial infarction (AMI) and stable coronary artery disease (SCAD) patients. These findings may help identify molecular mechanisms of acute thrombosis in AMI.
Area of Science:
- Cardiovascular Disease Research
- Proteomics
- Molecular Biology
Background:
- Acute myocardial infarction (AMI) is a primary cause of death in cardiovascular disease (CVD).
- The molecular mechanisms driving AMI pathogenesis remain largely unknown.
- Identifying individuals at risk for acute thrombosis is crucial.
Purpose of the Study:
- To identify differentially expressed proteins (DEPs) in coronary blood microparticles (MPs) between AMI and stable coronary artery disease (SCAD) patients using proteomics.
- To elucidate the biological functions and metabolic pathways associated with these DEPs.
- To understand the molecular mechanisms underlying AMI and acute thrombosis risk.
Main Methods:
- Proteomic analysis of coronary blood MPs from 5 AMI patients and 5 SCAD patients.
- Identification of differentially expressed proteins (DEPs).
- Gene Ontology (GO) and KEGG pathway enrichment analyses were performed on identified DEPs.
Main Results:
- 198 DEPs were identified between AMI and SCAD patients: 85 upregulated and 113 downregulated.
- GO enrichment analysis highlighted GDP binding and GTP binding in molecular function.
- KEGG pathway analysis indicated involvement in pantothenate and coenzyme A biosynthesis, starch/sucrose metabolism, and the AMPK signaling pathway.
Conclusions:
- The proteome of coronary MPs significantly differs between AMI and SCAD patients.
- Enriched GO terms and KEGG pathways suggest potential molecular mechanisms for AMI pathogenesis and acute thrombosis.
- This proteomic data may aid in identifying individuals at risk for acute thrombosis.
Background:
Acute myocardial infarction (AMI) is the leading cause of death for patients with cardiovascular disease (CVD). Although researchers have made substantial efforts to elucidate its pathogenesis, the molecular mechanisms underlying AMI remain unknown. The aim of this study was to use proteomics to identify differentially expressed proteins (DEPs) and the possible biological functions and metabolic pathways related to coronary blood microparticles (MPs) in patients with AMI and stable coronary artery disease (SCAD); this study will allow for the identification of individuals at risk of acute thrombosis.
Methods:
The study was performed on 5 AMI patients and 5 SCAD patients. DEPs were identified, and Gene Ontology (GO) enrichment and KEGG pathway enrichment analyzes were performed to determine the relative abundance and biological function of the significant DEPs that were identified in the present study.
Results:
The current analysis identified 198 DEPs in the coronary blood of AMI patients and SCAD patients, including 85 proteins that were significantly upregulated and 113 proteins that were significantly downregulated. GO enrichment analysis demonstrated that GDP binding and GTP binding were enriched in molecular function. Similarly, KEGG pathway enrichment analysis revealed that the identified proteins were involved in pantothenate and coenzyme A biosynthesis, starch and sucrose metabolism, and the AMPK signalling pathway.
Conclusions:
The proteome of coronary MPs differs between patients with AMI and patients with SCAD. In summary, the GO terms and KEGG pathways enriched by the DEPs may reflect the possible molecular mechanisms underlying the pathogenesis of acute thrombosis in patients with AMI.
More Related Videos
08:51Author Spotlight: Integrated Multi-Omics Analysis for Unveiling Multicellular Immune Signatures in Clinical Heart Attack Cohorts
Published on: September 20, 2024
10:03Coronary Progenitor Cells and Soluble Biomarkers in Cardiovascular Prognosis after Coronary Angioplasty
Published on: January 28, 2020
Related Concept Videos
01:20Blood Studies for Cardiovascular System I: Cardiac Biomarkers
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...
01:30Acute Coronary Syndrome III: Diagnostic Studies
01:30Acute Coronary Syndrome I: Introduction
01:19Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...
01:19Acute Coronary Syndrome II: Pathophysiology and Clinical Manifestations