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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
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Multiscale fluorescence imaging of living samples.

Yicong Wu1, Hari Shroff2,3

  • 1Laboratory of High-Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, 20892, USA. yicong.wu@nih.gov.

Histochemistry and Cell Biology
|August 29, 2022
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Summary

This study explores multiscale fluorescence imaging for living biological samples, detailing innovations and challenges. It synthesizes strategies to advance live imaging across different scales.

Keywords:
FluorescenceLive imagingMicroscopyMultiscale

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Area of Science:

  • Biological imaging
  • Microscopy techniques
  • Live-cell dynamics

Background:

  • Fluorescence microscopy is crucial for studying biological structure and function.
  • Imaging across multiple spatiotemporal scales presents unique challenges.
  • Multiscale fluorescence imaging of living samples is an understudied but vital area.

Purpose of the Study:

  • To survey recent innovations and applications in multiscale fluorescence imaging of living samples.
  • To discuss fundamental challenges associated with live multiscale imaging.
  • To synthesize general strategies for advancing this field.

Main Methods:

  • Review of recent literature on multiscale fluorescence imaging.
  • Analysis of successful case studies in live multiscale imaging.
  • Identification of common strategies and challenges.

Main Results:

  • Highlighting innovative techniques and applications in live multiscale imaging.
  • Detailed discussion of challenges in achieving high resolution across broad scales.
  • Presentation of successful examples demonstrating the power of the approach.

Conclusions:

  • Multiscale fluorescence imaging offers powerful insights into biological systems.
  • Addressing specific challenges can accelerate progress in live imaging.
  • Synthesized strategies aim to guide future research and development.