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Modulation of POPDC1 Expression by Phenothiazine and Trifluoperazine Suppress Colon Cancer Growth and Migration

Fiona Macniesia Thomas1, Suhaini Sudi1, Falah Abas Muhamad Salih1,2

  • 1Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu 88400, Sabah, Malaysia.

Asian Pacific Journal of Cancer Prevention : APJCP
|August 29, 2022
PubMed
Abstract

Insights

Calmodulin (CaM) antagonists, like PTZ and TFP, inhibit colon cancer cell proliferation and migration by downregulating PCNA and CD1. POPDC protein shows potential as a colon cancer biomarker, with CaM inhibition regulating POPDC1 expression for therapeutic strategies.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Colon cancer remains a significant global health challenge.
  • Calmodulin (CaM) plays a crucial role in various cellular processes, including proliferation and migration.
  • Understanding molecular targets for colon cancer therapy is essential.

Purpose of the Study:

  • To investigate the effects of CaM antagonists (PTZ, TFP) on colon cancer cell proliferation and migration.
  • To assess the impact of CaM antagonists on POPDC protein expression in colon cancer cells.
  • To explore the therapeutic potential of targeting CaM and POPDC1 in colon cancer.

Main Methods:

  • Utilized MTT assays to determine the 50% inhibitory concentration (IC50) of PTZ and TFP in multiple colon cancer cell lines.
  • Employed Western blot and immunocytochemistry to analyze the expression of proliferation markers (PCNA, CD1) and POPDC proteins.
  • Assessed cell migration using scratch wound-healing assays.

Main Results:

  • PTZ and TFP demonstrated dose-dependent inhibition of colon cancer cell growth.
  • Significant suppression of proliferation markers (PCNA, CD1) and cell migration was observed.
  • POPDC protein expression was notably reduced in all tested colon cancer cell lines, particularly SW480.
  • CaM antagonists were found to upregulate POPDC1 expression.

Conclusions:

  • CaM antagonists effectively suppress colon cancer cell proliferation by downregulating CD1 and PCNA.
  • POPDC protein emerges as a potential biomarker for colon cancer.
  • CaM antagonists can modulate POPDC1 expression, suggesting a therapeutic avenue.
  • Targeting POPDC1 via CaM inhibition presents a promising strategy for colon cancer treatment.
Keywords:
Colon cancerPOPDCcalmodulin antagonist

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