Related Experiment Video
Updated: Aug 30, 2025

A Simple and Efficient Method for In Vivo Cardiac-specific Gene Manipulation by Intramyocardial Injection in Mice
Published on: April 16, 2018
Tough Decoy-Mediated Cardiac Gene Suppression
1Division of Applied Medicine, School of Korean Medicine, Pusan National University, Yangsan, South Korea. khochangwon@pusan.ac.kr.
Abstract:
MicroRNA (miRNA) is a small, non-coding RNA molecule (~22 nucleotides) that acts as a post-transcriptional gene regulator, primarily by inhibiting the translation of target mRNA transcripts or affecting cell mRNA stability. Since miRNAs are comprehensively involved in gene regulation, their abnormalities are associated with various human diseases, including cardiovascular disease. Additionally, targeted inhibition of disease-related miRNAs and their targets should have therapeutic potential. Therefore, this chapter describes the experimental steps for targeted inhibition of specific miRNAs using adenoviral vectorized tough decoys that efficiently silence miRNA function in cardiac cells.
Related Concept Videos
In-vitro Mutagenesis
Gene Therapy

