Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

Clinical Applications of Epidermal Stem Cells

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Association between subjective symptoms and severity in patients with Fuchs endothelial corneal dystrophy.

Japanese journal of ophthalmology·2026

Same author

Crosstalk mediators implicated in the Stevens-Johnson Syndrome through gene regulatory network analysis.

BMC medical genomics·2026

Same author

Successful Cultivation of Oral Mucosal Epithelial Sheet Following Antifungal Therapy in Cases With Limbal Stem Cell Deficiency.

Cornea·2026

Same author

Intraocular Distribution and Aqueous-Vitreous Correlation of TGF-β Isoforms and GDF-15 in Retinal Diseases.

Current eye research·2026

Same author

Clinical performance of the multiplex solid-phase "Direct Strip PCR" for infectious uveitis: a multicenter diagnostic accuracy study.

Japanese journal of ophthalmology·2026

Same author

Peptide gel, CK2-085, maintains operative field visibility during surgery.

Scientific reports·2026

Search research articles

Related Experiment Video

Updated: Aug 30, 2025

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Published on: April 7, 2016

Current Advancements in Corneal Cell-Based Therapy.

Koji Kitazawa¹, Chie Sotozono¹, Shigeru Kinoshita²

¹Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.

Asia-Pacific Journal of Ophthalmology (Philadelphia, Pa.)

|August 30, 2022

Summary

Corneal cell therapy offers new hope for eye diseases. Rho-associated protein kinase inhibition enables functional corneal endothelial cell production for treating corneal failure.

More Related Videos

Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research

Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research

Published on: October 27, 2023

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Published on: September 12, 2014

Related Experiment Videos

Last Updated: Aug 30, 2025

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Published on: April 7, 2016

Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research

Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research

Published on: October 27, 2023

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Published on: September 12, 2014

Area of Science:

Ophthalmology
Regenerative Medicine
Stem Cell Biology

Background:

Corneal epithelial stem cells (CEpSCs) maintain ocular surface health.
Severe ocular surface diseases necessitate advanced cell-based therapies.
Corneal endothelial cells (CECs) have limited regenerative capacity in vivo.

Purpose of the Study:

To review historical advancements in corneal cell-based regenerative medicine.
To explore novel therapeutic strategies for corneal epithelial and endothelial diseases.
To highlight the potential of cultured CEC injection therapy.

Main Methods:

Review of key discoveries in corneal cell therapy.
Discussion of challenges in culturing functional corneal endothelial cells.
Exploration of Rho-associated protein kinase inhibition for CEC production.

Main Results:

Significant progress in in vivo and ex vivo ocular surface reconstruction.
Rho-associated protein kinase inhibition facilitates functional human CEC culture.
Cultured human CEC injection shows promise for corneal endothelial failure.

Conclusions:

Cell-based therapies are advancing for corneal epithelial and endothelial diseases.
Rho-associated protein kinase inhibition is a key breakthrough for CEC regeneration.
Corneal cell therapy represents a promising frontier in ophthalmology.