[Purification of radioactive sex hormones as receptor ligands]

A system (mobile phase) containing benzene:acetone:absolute ethanol 9:1:0.5, respectively, is described for thin-layer chromatography on Silufol plates of ligand receptors of sex steroid hormones 3H-5 alpha-androstan-3 beta, 17 beta-diol, 3H-estradiol-17 beta, 3H-5 alpha-dihydrotestosterone, 3H-methyltrienolone (R 1881), 3H-OPG 2058 and 3H-progesterone. A purity of commercially available 3H-steroids was decreased in storage, therefore they should be purified before use. The system described enabled to produce the 3H-steroids studied of high purity.