Hyperoxia by short-term promotes oxidative damage and mitochondrial dysfunction in rat brain

Oxygen (O₂) therapy is used as a therapeutic protocol to prevent or treat hypoxia. However, a high inspired fraction of O₂ (FIO₂) promotes hyperoxia, a harmful condition for the central nervous system (CNS). The present study evaluated parameters of oxidative stress and mitochondrial dysfunction in the brain of rats exposed to different FIO₂. Male Wistar rats were exposed to hyperoxia (FIO₂ 40 % and 60 %) compared to the control group (FIO₂ 21 %) for 2 h. Oxidative stress, neutrophilic infiltration, and mitochondrial respiratory chain enzymes were determined in the hippocampus, striatum, cerebellum, cortex, and prefrontal cortex after O₂ exposure. The animals exposed to hyperoxia showed increased lipid peroxidation, formation of carbonyl proteins, N/N concentration, and neutrophilic infiltration in some brain regions, like hippocampus, striatum, and cerebellum being the most affected. Furthermore, CAT activity and activity of mitochondrial enzyme complexes were also altered after exposure to hyperoxia. Rats exposed to hyperoxia showed increase in oxidative stress parameters and mitochondrial dysfunction in brain structures.