Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Biochemical differences between serotypes of Cryptococcus neoformans.

J E Bennett, K J Kwon-Chung, T S Theodore

    Sabouraudia
    |September 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids.

    Fungal genetics and biology : FG & B·2023
    Same author

    Inbred SJL mice recapitulate human resistance to <i>Cryptococcus</i> infection due to differential immune activation.

    mBio·2023
    Same author

    Novel angular naphthopyrone formation by Arp1p dehydratase involved in Aspergillus fumigatus melanin biosynthesis.

    Environmental microbiology reports·2021
    Same author

    <i>In Vivo</i> Efficacy of Olorofim against Systemic Scedosporiosis and Lomentosporiosis.

    Antimicrobial agents and chemotherapy·2021
    Same author

    Efficacy of Olorofim (F901318) against <i>Aspergillus fumigatus</i>, <i>A. nidulans</i>, and <i>A. tanneri</i> in Murine Models of Profound Neutropenia and Chronic Granulomatous Disease.

    Antimicrobial agents and chemotherapy·2019
    Same author

    Emerging <i>Aspergillus</i> Species Almost Exclusively Associated With Primary Immunodeficiencies.

    Open forum infectious diseases·2018
    Same journal

    Guanine plus cytosine content of the DNA of Coccidioides immitis.

    Sabouraudia·1985
    Same journal

    Canine mycotic keratoconjuntivitis caused by Acremonium kiliense.

    Sabouraudia·1985
    Same journal

    Scanning electron microscopic studies of Paracoccidioides brasiliensis in the yeast phase.

    Sabouraudia·1985
    Same journal

    Inhibition of growth and uptake of amino acids in the yeast, Candida parapsilosis, by ambruticin.

    Sabouraudia·1985
    Same journal

    Characteristics of the conidia produced by the mycelial form of Paracoccidioides brasiliensis.

    Sabouraudia·1985
    Same journal

    Mycotic pneumonia caused by Fusarium moniliforme in an alligator.

    Sabouraudia·1985
    See all related articles

    Cryptococcus neoformans serotypes B and C show distinct metabolic differences from serotypes A and D, including greater l-malic acid uptake and Guizotia seed agar greening. These findings aid in fungal isolate characterization.

    Area of Science:

    • Mycology
    • Microbial Physiology
    • Fungal Genetics

    Background:

    • Cryptococcus neoformans (C. neoformans) serotypes exhibit variations in morphology, distribution, and isolation sources.
    • Previous research indicated differences between serotypes B/C and A/D in C. neoformans.

    Purpose of the Study:

    • To investigate metabolic and biochemical differences between C. neoformans serotypes.
    • To identify novel characteristics that distinguish serotype B/C from serotype A/D.

    Main Methods:

    • Assessing radiolabeled l-malic acid uptake in different C. neoformans serotypes.
    • Evaluating the assimilation of various organic acids (l-malic, fumaric, succinic) by isolates.
    • Observing the characteristic greening reaction on Guizotia seed agar.

    Related Experiment Videos

    Main Results:

    • Serotypes B and C demonstrated approximately tenfold greater radiolabeled l-malic acid uptake compared to serotypes A and D.
    • Assimilation patterns of l-malic, fumaric, and succinic acids clearly separated serotypes B/C from A/D.
    • Greening of Guizotia seed agar was observed in 18/32 serotype B/C isolates but in none of the 91 serotype A/D isolates.
    • Creatinine assimilation was a distinguishing feature for serotype A alone, not aligning with other metabolic divisions.

    Conclusions:

    • Metabolic profiling, specifically l-malic acid uptake and Guizotia seed agar reaction, effectively differentiates C. neoformans serotypes B/C from A/D.
    • These biochemical markers provide valuable tools for fungal isolate classification and epidemiological studies.
    • Creatinine assimilation represents a unique characteristic of serotype A, distinct from the metabolic patterns observed in other serotypes.