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Related Experiment Video

Updated: Aug 30, 2025

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Cell Synchronization Techniques for Studying Mitosis.

Joanne D Hadfield^1,2,3, Sargun Sokhi^1,3, Gordon K Chan^4,5,6

¹Department of Oncology, University of Alberta, Edmonton, AB, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|August 31, 2022

Summary

This summary is machine-generated.

Synchronize cells in mitosis using reversible inhibitors like nocodazole and STLC. This method allows for synchronous release into the G1 phase, aiding cell cycle studies.

Keywords:

Cell cycle synchronization Double thymidine block MG132 Mitotic arrest Nocodazole STLC Timelapse imaging

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Last Updated: Aug 30, 2025

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

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Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy

Published on: November 29, 2017

Area of Science:

Cell Biology
Molecular Biology

Background:

Cell cycle synchronization is crucial for studying cell division dynamics.
Microtubule poisons, such as nocodazole, are common but can affect other cellular functions.
Alternative methods are needed for mitotic synchronization independent of microtubule disruption.

Purpose of the Study:

To describe and evaluate reversible methods for synchronizing cells in mitosis.
To present techniques applicable to Western blot and time-lapse imaging.
To enable synchronous release of mitotic cells into the G1 phase.

Main Methods:

Utilizing nocodazole, a microtubule inhibitor.
Employing STLC (Seneca Valley Virus-like Protein), a spindle assembly checkpoint inhibitor.
Combining thymidine block with MG132, a proteasome inhibitor.

Main Results:

Demonstrated successful mitotic arrest using nocodazole, STLC, and thymidine block/MG132 combination.
Confirmed the reversibility of these inhibitors, allowing for synchronous cell cycle progression.
Showcased the applicability of these methods for downstream analyses.

Conclusions:

Reversible inhibitors offer effective strategies for mitotic cell cycle synchronization.
These techniques provide alternatives to prolonged microtubule disruption.
Synchronized cell populations facilitate detailed studies of mitotic progression and cell cycle events.