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Updated: Aug 30, 2025

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Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization
Published on: August 16, 2015
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Analysis of Cell Cycle by Flow Cytometry
Hamid Maadi1, Mohammad Hasan Soheilifar2, Zhixiang Wang3
1Department of Oncology, Cross Cancer Institute, University of Alberta, Edmonton, AB, Canada. hamid.maadi@gmail.com.
Methods in Molecular Biology (Clifton, N.J.)
|August 31, 2022
Abstract:
The cell cycle of a cell is tightly controlled by several regulators. Dysregulation of cell cycle can lead to uncontrolled cell division which is one of the main characteristics of cancer cells. DNA content of a cell is changed during the cell cycle progression and can be measured by flow cytometry. In this chapter, we aim to provide a detailed protocol on how to analyze the cell cycle using flow cytometry.

