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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Aug 30, 2025

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Published on: August 16, 2015

Analysis of Cell Cycle by Flow Cytometry.

Hamid Maadi¹, Mohammad Hasan Soheilifar², Zhixiang Wang³

¹Department of Oncology, Cross Cancer Institute, University of Alberta, Edmonton, AB, Canada. hamid.maadi@gmail.com.

Methods in Molecular Biology (Clifton, N.J.)

|August 31, 2022

Summary

This summary is machine-generated.

This study details a flow cytometry protocol for analyzing cell cycle progression. Understanding cell cycle regulation is crucial for cancer research and therapeutic development.

Keywords:

Cell cycle DNA contents Flow cytometry Propidium iodide

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Related Experiment Videos

Last Updated: Aug 30, 2025

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Published on: August 16, 2015

Analysis of Cell Cycle Position in Mammalian Cells

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

Published on: May 22, 2012

Area of Science:

Cell Biology
Cancer Research
Biotechnology

Background:

Cell cycle progression is tightly regulated by specific proteins.
Dysregulation of the cell cycle is a hallmark of cancer, leading to uncontrolled cell proliferation.
Cellular DNA content fluctuates throughout the cell cycle.

Purpose of the Study:

To provide a comprehensive protocol for cell cycle analysis using flow cytometry.
To enable researchers to accurately measure DNA content and assess cell cycle distribution.
To facilitate the study of cell cycle regulators and their role in disease.

Main Methods:

Utilizing flow cytometry to measure cellular DNA content.
Staining cells with DNA-binding dyes (e.g., propidium iodide, DAPI).
Analyzing DNA histograms to determine cell populations in different cell cycle phases (G1, S, G2/M).

Main Results:

The protocol allows for precise quantification of cells in G1, S, and G2/M phases.
Flow cytometry effectively distinguishes cell cycle phases based on DNA content.
The method is robust for analyzing the impact of various treatments on cell cycle progression.

Conclusions:

Flow cytometry is a powerful and accessible technique for cell cycle analysis.
Accurate cell cycle profiling is essential for understanding cell proliferation and cancer biology.
This protocol serves as a valuable resource for researchers investigating cell cycle dynamics.