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Antigen-Down PEC Immunosensor for CYFRA21-1 Detection Based on Photocurrent Polarity Switching Strategy
Jinhuan Zhang1,2, Xiaodong Xue3, Yizhen Du3
1Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, Shandong, China.
Analytical Chemistry
|August 31, 2022
Summary
A novel photoelectrochemical (PEC) immunosensor detects cytokeratin 19 fragment 21-1 (CYFRA21-1) using signal polarity switching. This highly sensitive method offers a new approach for protein detection.
Area of Science:
- Electrochemistry
- Biosensing
- Materials Science
Background:
- Cytokeratin 19 fragment 21-1 (CYFRA21-1) is a significant biomarker for non-small cell lung cancer.
- Existing detection methods often lack sensitivity or require complex procedures.
- Photoelectrochemical (PEC) immunosensors offer potential for sensitive and rapid biomarker detection.
Purpose of the Study:
- To develop a novel antigen-down PEC immunosensor for CYFRA21-1 detection.
- To utilize a signal polarity switching strategy for enhanced sensitivity and reliability.
- To investigate the performance of 3,4,9,10-Perylene tetracarboxylic acid (PTCA) and SnS2 in the PEC system.
Main Methods:
- Fabrication of a PEC immunosensor using PTCA sensitized by SnS2 on an electrode.
- Immobilization of antibodies for CYFRA21-1 capture.
- Application of avidin-functionalized CuInS2 as a signal probe to induce photocurrent polarity switching.
- Detection of CYFRA21-1 through changes in photocurrent polarity.
Main Results:
- The developed PEC immunosensor demonstrated a signal polarity switching mechanism.
- Achieved high sensitivity with a linear detection range of 0.00001-500 ng·mL-1 for CYFRA21-1.
- Obtained a low detection limit of 3.5 fg·mL-1.
- Exhibited excellent stability, selectivity, and repeatability.
Conclusions:
- The proposed antigen-down PEC immunosensor with signal polarity switching offers a highly sensitive and reliable method for CYFRA21-1 detection.
- The combination of PTCA, SnS2, and avidin-functionalized CuInS2 enhances sensor performance.
- This approach provides a promising new avenue for the detection of CYFRA21-1 and other protein biomarkers.
