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miR-100-5p Promotes Epidermal Stem Cell Proliferation through Targeting MTMR3 to Activate PIP3/AKT and ERK Signaling
Stem Cells International
|September 1, 2022
Summary
Human adipose-derived stem cell exosomes (ADSC-Exos) promote skin stem cell proliferation via miR-100-5p, which targets MTMR3. This mechanism enhances wound healing and skin tissue engineering applications.
Area of Science:
- Regenerative Medicine
- Stem Cell Biology
- Molecular Biology
Background:
- Skin epidermal stem cells (EpSCs) are crucial for wound healing and skin tissue engineering.
- Human adipose-derived stem cell exosomes (ADSC-Exos) enhance EpSC proliferation, but the mechanism is unclear.
Purpose of the Study:
- Investigate the role of miR-100-5p from ADSC-Exos in human EpSC proliferation.
- Elucidate the underlying molecular mechanisms involving miR-100-5p and its target MTMR3.
Main Methods:
- Utilized MTT and BrdU assays to assess EpSC proliferation.
- Performed cell cycle analysis, RNA interference, and Western blotting.
- Investigated the effects of ADSC-Exos and miR-100-5p in cultured human skin tissues.
Main Results:
- miR-100-5p mimic transfection promoted EpSC proliferation and altered cell cycle distribution.
- Identified Myotubularin-related protein 3 (MTMR3) as a direct target of miR-100-5p.
- miR-100-5p downregulated MTMR3, increasing PIP3 levels and activating AKT/ERK signaling, leading to enhanced EpSC proliferation.
Conclusions:
- miR-100-5p promotes EpSC proliferation by downregulating MTMR3, elevating PIP3, and activating AKT/ERK pathways.
- miR-100-5p-enriched ADSC-Exos show therapeutic potential for skin wound treatment and EpSC expansion for tissue engineering.
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