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Letter to the Editor relating to Clin Chem Lab Med 2022;60(9):1365-72
Xiang Sun1, Qin Wang1, Qun Shi1
1Department of Clinical Laboratory, Shanghai Fengxian District Hospital of Traditional Chinese Medicine, Shanghai, P.R. China.
Clinical Chemistry and Laboratory Medicine
|September 2, 2022
Abstract
No abstract available in PubMed .
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Overview
Enzyme-Linked Immunosorbent Assay
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

