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Related Experiment Video

Updated: Aug 30, 2025

An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
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Visualization of intercellular cargo transfer using upconverting nanoparticles.

Yeongchang Goh1, Jongwoo Kim2, Hye Sun Park3

  • 1Department of Chemistry, Gwangju Institute of Science and Technology (GIST), Gwangju, South Korea. ktlee@gist.ac.kr.

Nanoscale
|September 2, 2022
PubMed
Summary

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Nanoscale Epigenetic Profiling of Colorectal Cancer Cell-Derived Exosomes via Single-Vesicle Nanoscopy.

Small methods·2025
This summary is machine-generated.

This study visualizes intercellular cargo transfer using dual-color imaging with upconverting nanoparticles (UCNPs). Extracellular vesicles (EVs) were tracked in live cells, revealing mechanisms of cell-cell communication.

Area of Science:

  • Cell Biology
  • Nanotechnology
  • Biophysics

Background:

  • Cell-cell communication is vital for biological processes like differentiation and immune response.
  • Extracellular vesicles (EVs) mediate intercellular communication by transferring cargo molecules.
  • Understanding EV-mediated transport is crucial for deciphering cellular regulation.

Purpose of the Study:

  • To demonstrate a novel dual-color imaging technique for observing intercellular cargo transfer.
  • To visualize real-time, long-term single-vesicle tracking of EVs in live cells.
  • To investigate both contact-dependent and contact-independent signaling pathways.

Main Methods:

  • Utilized upconverting nanoparticles (UCNPs) for dual-color imaging.
  • Employed real-time, long-term single-vesicle tracking.

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  • Observed intercellular transport in live HeLa cells.

    • Main Results:

    • Successfully visualized intercellular cargo transfer via UCNPs.
    • Demonstrated real-time tracking of EVs in both contact-dependent and independent signaling.
    • Showed that endocytosed UCNPs can be transmitted via fluorescently labeled EVs.

    Conclusions:

    • Dual-color imaging with UCNPs provides a powerful tool for studying intercellular communication.
    • This technique enables detailed observation of EV-mediated cargo transfer.
    • The findings offer insights into the mechanisms of cell-cell signaling.