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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Aug 29, 2025

Flow Cytometry-Based Quantification and Analysis of Myocardial B-Cells

Flow Cytometry-Based Quantification and Analysis of Myocardial B-Cells

Published on: August 17, 2022

Flow Cytometry-Based Quantification and Analysis of Myocardial B-cells.

Kevin C Bermea¹, Sylvie T Rousseau¹, Luigi Adamo²

¹Division of Cardiology, Department of Medicine, The Johns Hopkins University School of Medicine.

Journal of Visualized Experiments : Jove

|September 5, 2022

Summary

Accurately quantifying B-lymphocytes in the heart requires a standardized method. This study optimizes B-cell detection by addressing perfusion and digestion variables, enabling reproducible analysis of myocardial B-cells.

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Last Updated: Aug 29, 2025

Flow Cytometry-Based Quantification and Analysis of Myocardial B-Cells

Flow Cytometry-Based Quantification and Analysis of Myocardial B-Cells

Published on: August 17, 2022

Isolation and Identification of Extravascular Immune Cells of the Heart

Isolation and Identification of Extravascular Immune Cells of the Heart

Published on: August 23, 2018

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Published on: May 5, 2017

Area of Science:

Immunology
Cardiovascular Biology
Cell Biology

Background:

B-lymphocytes are increasingly recognized for their roles in heart function and injury response.
Existing literature presents conflicting data on the prevalence and changes in myocardial B-cells following injury.
Discrepancies may arise from methodological variations in assessing B-cell populations within the heart.

Purpose of the Study:

To develop a reproducible method for quantifying B-lymphocytes in the murine heart.
To address critical variables affecting B-cell recovery, specifically perfusion and digestion.
To enable accurate differentiation between intravascular and extravascular myocardial B-cells.

Main Methods:

Optimization of organ perfusion techniques to minimize B-cell loss.
Refinement of tissue digestion protocols for efficient B-cell isolation.
Flow cytometry-based analysis for precise enumeration of myocardial B-cells.
Distinguishing between intravascular and extravascular B-cell populations.

Main Results:

An optimized protocol was established for reproducible B-cell quantification in murine hearts.
The method accounts for perfusion and digestion variables, crucial for accurate B-cell recovery.
Researchers can now reliably distinguish between intravascular and extravascular B-cells in myocardial tissue.

Conclusions:

A standardized, optimized protocol is essential for harmonizing research on myocardial B-cells.
This method facilitates accurate assessment of B-cell prevalence and distribution in the heart.
Advancing the understanding of B-cell interactions in cardiac physiology and pathology is now more feasible.